Next Article in Journal
Bacteriophage-Derived Vectors for Targeted Cancer Gene Therapy
Previous Article in Journal
Identification of a Novel Human Rhinovirus C Type by Antibody Capture VIDISCA-454
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(1), 252-267; doi:10.3390/v7010252

A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis

1
Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep, 0033 Oslo, Norway
2
MSD Animal Health Norway, Thormøhlensgate 55, N-5008 Bergen, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Curt Hagedorn
Received: 4 November 2014 / Accepted: 13 January 2015 / Published: 19 January 2015
(This article belongs to the Section Antivirals & Vaccines)
View Full-Text   |   Download PDF [1536 KB, uploaded 12 May 2015]   |  

Abstract

Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV), was used for in vitro and in vivo expression of IPN virus proteins. The large open reading frame of segment A, encoding the polyprotein NH2-pVP2-VP4-VP3-COOH, as well as pVP2, were cloned and expressed by the SAV replicon in Chinook salmon embryo cells (CHSE-214) and epithelioma papulosum cyprini (EPC) cells. The replicon constructs pSAV/polyprotein (pSAV/PP) and pSAV/pVP2 were used to immunize Atlantic salmon (Salmo salar) by a single intramuscular injection and tested in a subsequent IPN virus (IPNV) challenge trial. A low to moderate protection against IPN was observed in fish immunized with the replicon vaccine that encoded the pSAV/PP, while the pSAV/pVP2 construct was not found to induce protection. View Full-Text
Keywords: infectious pancreatic necrosis; Atlantic salmon; vaccination; alphavirus replicon infectious pancreatic necrosis; Atlantic salmon; vaccination; alphavirus replicon
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Abdullah, A.; Olsen, C.M.; Hodneland, K.; Rimstad, E. A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis. Viruses 2015, 7, 252-267.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top