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Viruses 2012, 4(5), 777-799; doi:10.3390/v4050777
Review

Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly

1
, 2,*  and 1,3,*
Received: 26 March 2012; Accepted: 24 April 2012 / Published: 4 May 2012
(This article belongs to the Special Issue Frontiers in Imaging)
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Abstract: Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells and is expected to become even more indispensable in the future. Here, we describe different fluorescence labeling strategies that have been used to label HIV-1 for live cell imaging and the fluorescence based methods used to visualize individual aspects of virus-cell interactions. This review presents an overview of experimental methods and recent experiments that have employed quantitative microscopy in order to elucidate the dynamics of late stages in the HIV-1 replication cycle. This includes cytosolic interactions of the main structural protein, Gag, with itself and the viral RNA genome, the recruitment of Gag and RNA to the plasma membrane, virion assembly at the membrane and the recruitment of cellular proteins involved in HIV-1 release to the nascent budding site.
Keywords: HIV; assembly; fluorescence; microscopy; ESCRT, live-cell imaging HIV; assembly; fluorescence; microscopy; ESCRT, live-cell imaging
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Baumgärtel, V.; Müller, B.; Lamb, D.C. Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly. Viruses 2012, 4, 777-799.

AMA Style

Baumgärtel V, Müller B, Lamb DC. Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly. Viruses. 2012; 4(5):777-799.

Chicago/Turabian Style

Baumgärtel, Viola; Müller, Barbara; Lamb, Don C. 2012. "Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly." Viruses 4, no. 5: 777-799.



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