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Viruses 2012, 4(5), 761-776; doi:10.3390/v4050761
Article

Conserved Structural Motifs at the C-Terminus of Baculovirus Protein IE0 are Important for its Functions in Transactivation and Supporting hr5-mediated DNA Replication

1
, 2
 and 1,*
1 Entomology Department, Institute of Plant Protection, The Volcani Center, POB 6, Bet Dagan 50250, Israel 2 Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Ministry of Education), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
* Author to whom correspondence should be addressed.
Received: 9 February 2012 / Revised: 23 April 2012 / Accepted: 27 April 2012 / Published: 4 May 2012
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Abstract

IE0 and IE1 are transactivator proteins of the most studied baculovirus, the Autographa californica multiple nucleopolyhedrovirus (AcMNPV). IE0 is a 72.6 kDa protein identical to IE1 with the exception of its 54 N-terminal amino acid residues. To gain some insight about important structural motifs of IE0, we expressed the protein and C‑terminal mutants of it under the control of the Drosophila heat shock promoter and studied the transactivation and replication functions of the transiently expressed proteins. IE0 was able to promote replication of a plasmid bearing the hr5 origin of replication of AcMNPV in transient transfections with a battery of eight plasmids expressing the AcMNPV genes dnapol, helicase, lef-1, lef-2, lef-3, p35, ie-2 and lef-7. IE0 transactivated expression of the baculovirus 39K promoter. Both functions of replication and transactivation were lost after introduction of selected mutations at the basic domain II and helix-loop-helix conserved structural motifs in the C-terminus of the protein. These IE0 mutants were unable to translocate to the cell nucleus. Our results point out the important role of some structural conserved motifs to the proper functioning of IE0.
Keywords: IE0 transactivator; baculovirus; mutagenesis; functions IE0 transactivator; baculovirus; mutagenesis; functions
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Luria, N.; Lu, L.; Chejanovsky, N. Conserved Structural Motifs at the C-Terminus of Baculovirus Protein IE0 are Important for its Functions in Transactivation and Supporting hr5-mediated DNA Replication. Viruses 2012, 4, 761-776.

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