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• Gardinassi, LGustavo Araujo
• Simas, PVitor Marques
• Gomes, DElias
• Bonfim, CMeasso do
• Nogueira, FCavassan
• Garcia, GRocha
• Carareto, CMárcia Aparecida
• Rahal, P
• Souza, FPereira de
• [+] on Google Scholar
• Gardinassi, LGustavo Araujo
• Simas, PVitor Marques
• Gomes, DElias
• Bonfim, CMeasso do
• Nogueira, FCavassan
• Garcia, GRocha
• Carareto, CMárcia Aparecida
• Rahal, P
• Souza, FPereira de
• [+] on PubMed
• Gardinassi, LGustavo Araujo
• Simas, PVitor Marques
• Gomes, DElias
• Bonfim, CMeasso do
• Nogueira, FCavassan
• Garcia, GRocha
• Carareto, CMárcia Aparecida
• Rahal, P
• Souza, FPereira de

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Viruses 2012, 4(11), 2432-2447; doi:10.3390/v4112432

Article

Diversity and Adaptation of Human Respiratory Syncytial Virus Genotypes Circulating in Two Distinct Communities: Public Hospital and Day Care Center

Luiz Gustavo Araujo Gardinassi ^3,† , Paulo Vitor Marques Simas ^1,† , Deriane Elias Gomes ^2,† , Caroline Measso do Bonfim ¹ , Felipe Cavassan Nogueira ¹ , Gustavo Rocha Garcia ³ , Claudia Márcia Aparecida Carareto ¹ , Paula Rahal ¹  and Fátima Pereira de Souza ^2,*

¹ Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas de São José do Rio Preto, SP. Departamento de Biologia - Rua Cristóvão Colombo, 2265, Jardim Nazareth – Cep: 15054-000, Brazil ² Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas de São José do Rio Preto, SP. Departamento de Física - Rua Cristóvão Colombo, 2265, Jardim Nazareth – Cep: 15054-000, Brazil ³ Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, SP. Departamento de Bioquímica e Imunologia – Av. dos Bandeirantes, 3900 Monte Alegre – Cep: 14049-900, Brazil ^† These authors contributed equally to this work.

* Author to whom correspondence should be addressed.

Received: 31 August 2012; in revised form: 16 October 2012 / Accepted: 17 October 2012 / Published: 24 October 2012

(This article belongs to the Special Issue Pneumoviruses and Metapneumoviruses)

Download PDF Full-Text [467 KB, uploaded 24 October 2012 14:58 CEST]

Abstract: HRSV is one of the most important pathogens causing acute respiratory tract diseases as bronchiolitis and pneumonia among infants. HRSV was isolated from two distinct communities, a public day care center and a public hospital in São José do Rio Preto – SP, Brazil. We obtained partial sequences from G gene that were used on phylogenetic and selection pressure analysis. HRSV accounted for 29% of respiratory infections in hospitalized children and 7.7% in day care center children. On phylogenetic analysis of 60 HRSV strains, 48 (80%) clustered within or adjacent to the GA1 genotype; GA5, NA1, NA2, BA-IV and SAB1 were also observed. SJRP GA1 strains presented variations among deduced amino acids composition and lost the potential O-glycosilation site at amino acid position 295, nevertheless this resulted in an insertion of two potential O-glycosilation sites at positions 296 and 297. Furthermore, a potential O-glycosilation site insertion, at position 293, was only observed for hospital strains. Using SLAC and MEME methods, only amino acid 274 was identified to be under positive selection. This is the first report on HRSV circulation and genotypes classification derived from a day care center community in Brazil.

Keywords: respiratory syncytial virus; attachment protein (G); genetic variability; O-glycosilation; selection pressure

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