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Viruses 2012, 4(11), 2432-2447; doi:10.3390/v4112432
Article

Diversity and Adaptation of Human Respiratory Syncytial Virus Genotypes Circulating in Two Distinct Communities: Public Hospital and Day Care Center

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Received: 31 August 2012; in revised form: 16 October 2012 / Accepted: 17 October 2012 / Published: 24 October 2012
(This article belongs to the Special Issue Pneumoviruses and Metapneumoviruses)
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Abstract: HRSV is one of the most important pathogens causing acute respiratory tract diseases as bronchiolitis and pneumonia among infants. HRSV was isolated from two distinct communities, a public day care center and a public hospital in São José do Rio Preto – SP, Brazil. We obtained partial sequences from G gene that were used on phylogenetic and selection pressure analysis. HRSV accounted for 29% of respiratory infections in hospitalized children and 7.7% in day care center children. On phylogenetic analysis of 60 HRSV strains, 48 (80%) clustered within or adjacent to the GA1 genotype; GA5, NA1, NA2, BA-IV and SAB1 were also observed. SJRP GA1 strains presented variations among deduced amino acids composition and lost the potential O-glycosilation site at amino acid position 295, nevertheless this resulted in an insertion of two potential O-glycosilation sites at positions 296 and 297. Furthermore, a potential O-glycosilation site insertion, at position 293, was only observed for hospital strains. Using SLAC and MEME methods, only amino acid 274 was identified to be under positive selection. This is the first report on HRSV circulation and genotypes classification derived from a day care center community in Brazil.
Keywords: respiratory syncytial virus; attachment protein (G); genetic variability; O-glycosilation; selection pressure respiratory syncytial virus; attachment protein (G); genetic variability; O-glycosilation; selection pressure
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Gardinassi, L.G.A.; Simas, P.V.M.; Gomes, D.E.; Bonfim, C.M.; Nogueira, F.C.; Garcia, G.R.; Carareto, C.M.A.; Rahal, P.; Souza, F.P. Diversity and Adaptation of Human Respiratory Syncytial Virus Genotypes Circulating in Two Distinct Communities: Public Hospital and Day Care Center. Viruses 2012, 4, 2432-2447.

AMA Style

Gardinassi LGA, Simas PVM, Gomes DE, Bonfim CM, Nogueira FC, Garcia GR, Carareto CMA, Rahal P, Souza FP. Diversity and Adaptation of Human Respiratory Syncytial Virus Genotypes Circulating in Two Distinct Communities: Public Hospital and Day Care Center. Viruses. 2012; 4(11):2432-2447.

Chicago/Turabian Style

Gardinassi, Luiz G.A.; Simas, Paulo V.M.; Gomes, Deriane E.; Bonfim, Caroline M.; Nogueira, Felipe C.; Garcia, Gustavo R.; Carareto, Claudia M.A.; Rahal, Paula; Souza, Fátima P. 2012. "Diversity and Adaptation of Human Respiratory Syncytial Virus Genotypes Circulating in Two Distinct Communities: Public Hospital and Day Care Center." Viruses 4, no. 11: 2432-2447.


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