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Construction and Testing of orfA +/- FIV Reporter Viruses
Mayo Clinic, Department of Molecular Medicine, Guggenheim 18-11A, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
Mayo Clinic, Division of Infectious Diseases, Guggenheim 18-11A, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
* Author to whom correspondence should be addressed.
Received: 21 December 2011; in revised form: 15 January 2012 / Accepted: 16 January 2012 / Published: 23 January 2012
Abstract: Single cycle reporter viruses that preserve the majority of the HIV-1 genome, long terminal repeat-promoted transcription and Rev-dependent structural protein expression are useful for investigating the viral life cycle. Reporter viruses that encode the viral proteins in cis in this way have been lacking for feline immunodeficiency virus (FIV), where the field has used genetically minimized transfer vectors with viral proteins supplied in trans. Here we report construction and use of a panel of single cycle FIV reporter viruses that express fluorescent protein markers. The viruses can be produced to high titer using human cell transfection and can transduce diverse target cells. To illustrate utility, we tested versions that are (+) and (-) for OrfA, an FIV accessory protein required for replication in primary lymphocytes and previously implicated in down-regulation of the primary FIV entry receptor CD134. We observed CD134 down-regulation after infection with or without OrfA, and equivalent virion production as well. These results suggest a role for FIV proteins besides Env or OrfA in CD134 down-regulation.
Keywords: FIV; lentivirus; reporter virus; OrfA
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MDPI and ACS Style
Fadel, H.J.; Saenz, D.T.; Poeschla, E.M. Construction and Testing of orfA +/- FIV Reporter Viruses. Viruses 2012, 4, 184-199.
Fadel HJ, Saenz DT, Poeschla EM. Construction and Testing of orfA +/- FIV Reporter Viruses. Viruses. 2012; 4(1):184-199.
Fadel, Hind J.; Saenz, Dyana T.; Poeschla, Eric M. 2012. "Construction and Testing of orfA +/- FIV Reporter Viruses." Viruses 4, no. 1: 184-199.