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Primed for Discovery: Atomic-Resolution Cryo-EM Structure of a Reovirus Entry Intermediate
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
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Received: 2 June 2010; in revised form: 10 June 2010 / Accepted: 14 June 2010 / Published: 15 June 2010
(This article belongs to the Section Editorial
Abstract: A recently solved structure of the aquareovirus virion (Zhang, X; Jin, L.; Fang, Q; Hui, W.H.; Zhou Z.H. 3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry. Cell 2010, 141, 472-482 ) provides new insights into the order of entry events, as well as confirming and refining several aspects of the entry mechanism, for aquareovirus and the related orthoreovirus. In particular, the structure provides evidence of a defined order for the progressive proteolytic cleavages of myristoylated penetration protein VP5 that prime the virion for membrane penetration. These observations reinforce the concept that, much like enveloped viruses, nonenveloped virions often undergo priming events that lead to a meta-stable state, preparing the virus for membrane penetration under the appropriate circumstances. In addition, this and other recent studies highlight the increasing power of electron cryomicroscopy to analyze large, geometrically regular structures, such as icosahedral viruses, at atomic resolution.
Keywords: aquareovirus; orthoreovirus; Reoviridae; electron cryomicroscopy; nonenveloped virus; virus entry; myristoyl; autoproteolysis
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Trask, S.D.; Guglielmi, K.M.; Patton, J.T. Primed for Discovery: Atomic-Resolution Cryo-EM Structure of a Reovirus Entry Intermediate. Viruses 2010, 2, 1340-1346.
Trask SD, Guglielmi KM, Patton JT. Primed for Discovery: Atomic-Resolution Cryo-EM Structure of a Reovirus Entry Intermediate. Viruses. 2010; 2(6):1340-1346.
Trask, Shane D.; Guglielmi, Kristen M.; Patton, John T. 2010. "Primed for Discovery: Atomic-Resolution Cryo-EM Structure of a Reovirus Entry Intermediate." Viruses 2, no. 6: 1340-1346.