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Viruses 2018, 10(5), 223; https://doi.org/10.3390/v10050223

Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort

1
Molecular Biology Laboratory, LMCBM, SPC, HEM—Centro Hospitalar Lisboa Ocidental, 1349-019 Lisboa, Portugal
2
Global Health and Tropical Medicine (GHTM), Unit of Medical Microbiology, Instituto de Higiene e Medicina Tropical (IHMT), Universidade NOVA de Lisboa (UNL), 1349-008 Lisboa, Portugal
3
Serviço de Doenças Infeciosas, HEM—Centro Hospitalar Lisboa Ocidental, 1349-019 Lisboa, Portugal
4
Centro de Investigação Interdisciplinar Egas Moniz, CiiEM, ISCSEM, 2829-511 Almada, Portugal
*
Author to whom correspondence should be addressed.
Received: 11 April 2018 / Revised: 20 April 2018 / Accepted: 22 April 2018 / Published: 26 April 2018
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Abstract

This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04). View Full-Text
Keywords: hepatitis C virus; direct-acting antivirals; resistance-associated substitutions; NS5A; NS5B hepatitis C virus; direct-acting antivirals; resistance-associated substitutions; NS5A; NS5B
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Brandão, R.; Marcelino, R.; Gonçalves, F.; Diogo, I.; Carvalho, A.; Cabanas, J.; Costa, I.; Brogueira, P.; Ventura, F.; Miranda, A.; Mansinho, K.; Gomes, P. Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort. Viruses 2018, 10, 223.

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