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Forests 2016, 7(11), 274; doi:10.3390/f7110274

Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line

1
Departamento de Ecologıía y Genética Forestal, INIA-CIFOR, Unidad Mixta de Genómica y Ecofisiología Forestal, INIA/UPM, Madrid 28040, Spain
2
Departamento de Biología Vegetal, Facultad de Farmacia, ERI BiotecMed, Universidad de Valencia, Burjassot, Valencia 46100, Spain
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: John MacKay and Stephen P. DiFazio
Received: 4 September 2016 / Revised: 4 November 2016 / Accepted: 9 November 2016 / Published: 12 November 2016
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Abstract

Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of this, chromosomal stability of the callus and/or somatic embryos should also be assessed. To this end, chromosome counting, flow cytometry and genotyping using microsatellites have been reported. Here, we present an overview of the work done in conifers, with special emphasis on the production of a haploid cell line in maritime pine (Pinus pinaster L.) and the use of a set of molecular markers, which includes Single Nucleotide Polymorphisms (SNPs) and microsatellites or Single Sequence Repeats (SSRs), to validate chromosomal integrity confirming the presence of all chromosomic arms. View Full-Text
Keywords: genetic stability; genome sequencing; haploid tissue; molecular markers; Pinus pinaster genetic stability; genome sequencing; haploid tissue; molecular markers; Pinus pinaster
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MDPI and ACS Style

Cabezas, J.A.; Morcillo, M.; Vélez, M.D.; Díaz, L.; Segura, J.; Cervera, M.T.; Arrillaga, I. Haploids in Conifer Species: Characterization and Chromosomal Integrity of a Maritime Pine Cell Line. Forests 2016, 7, 274.

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