Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Synthesis
2.2. Cell Culture and Cell Immobilisation
2.3. Scaffold Fabrication
2.4. Hard-Phase Evaluation
2.4.1. FTIR
2.4.2. Contact Angle Measurement
2.4.3. Cell Behaviour on Different PCL-PEG Blends
2.5. Hard-Phase Scaffold Characterisation
2.5.1. Strut Size and Pore Size
2.5.2. Porosity
2.5.3. Mechanical Testing
2.5.4. Degradation Study
2.5.5. Scanning Electron Microscopy (SEM)
2.6. Hard-Soft Phase Scaffolds: In-Vitro Characterisation
2.6.1. Optical Microscopy
2.6.2. Cell Viability
2.6.3. Cell Adhesion
2.7. Statistical Analysis
3. Results and Discussion
3.1. Hard-Phase Evaluation
3.1.1. FTIR
3.1.2. Contact Angle Measurements
3.1.3. Cell Adhesion and Cell Viability
3.2. Hard-Phase Scaffold Characterisation
3.2.1. Scaffold Design Data
3.2.2. Degradation Study: Mass Loss
3.2.3. Scanning Electron Microscopy
3.2.4. Mechanical Testing
3.3. Hard-Soft Phase Scaffolds: In-Vitro Characterisation
3.3.1. Cell Viability
3.3.2. Optical Microscopy
3.3.3. Cell Distribution and Cell Morphology
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Design (Lines Per Layer) | Material | Strut Width (µm) | Pore Size (µm) | Porosity (%) |
---|---|---|---|---|
14 | PCL | 358 ± 15 | 401 ± 20 | 41 ± 3 |
14 | PCL-PEG 8020 | 425 ± 118 | 318 ± 123 | 33 ± 13 |
14 | PCL-PEG 7030 | 313 ± 73 | 434 ± 67 | 52 ± 9 |
10 | PCL | 313 ± 72 | 801 ± 67 | 65 ± 5 |
10 | PCL-PEG 8020 | 380 ± 79 | 712 ± 76 | 46 ± 5 |
10 | PCL-PEG 7030 | 307 ± 84 | 794 ± 104 | 65 ± 10 |
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Zehnder, T.; Freund, T.; Demir, M.; Detsch, R.; Boccaccini, A.R. Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering. Materials 2016, 9, 887. https://doi.org/10.3390/ma9110887
Zehnder T, Freund T, Demir M, Detsch R, Boccaccini AR. Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering. Materials. 2016; 9(11):887. https://doi.org/10.3390/ma9110887
Chicago/Turabian StyleZehnder, Tobias, Tim Freund, Merve Demir, Rainer Detsch, and Aldo R. Boccaccini. 2016. "Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering" Materials 9, no. 11: 887. https://doi.org/10.3390/ma9110887