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Materials 2013, 6(12), 5857-5869; doi:10.3390/ma6125857
Article

Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium

1
, 2,* , 1
, 3
, 4
 and 1,*
1 Department of Frontier Materials, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan 2 Center for Fostering Young and Innovative Researchers, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan 3 Dental Materials Lab, Dental Clinic 1, University of Erlangen-Nuremberg, Glueckstrasse 11, Erlangen 91054, Germany 4 Biometals Group, Biomaterials Unit, Nano-Bio Field, International Center for Materials Nanoarchitechtonics (MANA), National Institute for Materials Science (NIMS), 1-1, Namiki, Tsukuba 305-0044, Japan
* Authors to whom correspondence should be addressed.
Received: 25 October 2013 / Revised: 27 November 2013 / Accepted: 10 December 2013 / Published: 12 December 2013
(This article belongs to the Special Issue Ceramics for Healthcare 2013)
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Abstract

Poly(l-lactic acid)-based films which include 60 wt % of vaterite (V) or siloxane-containing vaterite (SiV) were coated on a pure magnesium substrate, denoted by PLLA/V or PLLA/SiV, respectively, to suppress early corrosion and improve its cytocompatibility. Both coating films adhered to the Mg substrate with 2.3–2.8 MPa of tensile bonding strength. Soaking test for 7 days in α-modified minimum essential medium revealed that the morphological instability of the PLLA/V film caused a higher amount of Mg2+ ion to be released from the coating sample. On the other hand, in the case of the coating with the PLLA/SiV film, no morphological change even after the soaking test was observed, owing to the suppression of the degradation rate. In cell culture tests, the proliferation of mouse osteoblast-like cell (MC3T3-E1) was significantly enhanced by both coatings, in comparison with the uncoated magnesium substrate. The cell morphology revealed that a few less-spread cells were observed on the PLLA/V film, while more elongated cells were done on the PLLA/SiV film. The cells on the PLLA/SiV film exhibited an extremely higher alkaline phosphatase activity after 21 days of incubation than that on the PLLA/V one. The PLLA/SiV film suppressed the early corrosion and enhanced cytocompatibility on metallic magnesium.
Keywords: coating; magnesium; poly(l-lactic acid); vaterite (calcium carbonate); composite coating; magnesium; poly(l-lactic acid); vaterite (calcium carbonate); composite
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yamada, S.; Maeda, H.; Obata, A.; Lohbauer, U.; Yamamoto, A.; Kasuga, T. Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium. Materials 2013, 6, 5857-5869.

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