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Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium
Department of Frontier Materials, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan
Center for Fostering Young and Innovative Researchers, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan
Dental Materials Lab, Dental Clinic 1, University of Erlangen-Nuremberg, Glueckstrasse 11, Erlangen 91054, Germany
Biometals Group, Biomaterials Unit, Nano-Bio Field, International Center for Materials Nanoarchitechtonics (MANA), National Institute for Materials Science (NIMS), 1-1, Namiki, Tsukuba 305-0044, Japan
* Authors to whom correspondence should be addressed.
Received: 25 October 2013; in revised form: 27 November 2013 / Accepted: 10 December 2013 / Published: 12 December 2013
Abstract: Poly(l-lactic acid)-based films which include 60 wt % of vaterite (V) or siloxane-containing vaterite (SiV) were coated on a pure magnesium substrate, denoted by PLLA/V or PLLA/SiV, respectively, to suppress early corrosion and improve its cytocompatibility. Both coating films adhered to the Mg substrate with 2.3–2.8 MPa of tensile bonding strength. Soaking test for 7 days in α-modified minimum essential medium revealed that the morphological instability of the PLLA/V film caused a higher amount of Mg2+ ion to be released from the coating sample. On the other hand, in the case of the coating with the PLLA/SiV film, no morphological change even after the soaking test was observed, owing to the suppression of the degradation rate. In cell culture tests, the proliferation of mouse osteoblast-like cell (MC3T3-E1) was significantly enhanced by both coatings, in comparison with the uncoated magnesium substrate. The cell morphology revealed that a few less-spread cells were observed on the PLLA/V film, while more elongated cells were done on the PLLA/SiV film. The cells on the PLLA/SiV film exhibited an extremely higher alkaline phosphatase activity after 21 days of incubation than that on the PLLA/V one. The PLLA/SiV film suppressed the early corrosion and enhanced cytocompatibility on metallic magnesium.
Keywords: coating; magnesium; poly(l-lactic acid); vaterite (calcium carbonate); composite
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MDPI and ACS Style
Yamada, S.; Maeda, H.; Obata, A.; Lohbauer, U.; Yamamoto, A.; Kasuga, T. Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium. Materials 2013, 6, 5857-5869.
Yamada S, Maeda H, Obata A, Lohbauer U, Yamamoto A, Kasuga T. Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium. Materials. 2013; 6(12):5857-5869.
Yamada, Shinya; Maeda, Hirotaka; Obata, Akiko; Lohbauer, Ulrich; Yamamoto, Akiko; Kasuga, Toshihiro. 2013. "Cytocompatibility of Siloxane-Containing Vaterite/Poly(l-lactic acid) Composite Coatings on Metallic Magnesium." Materials 6, no. 12: 5857-5869.