Materials 2012, 5(11), 2306-2325; doi:10.3390/ma5112306

Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study

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Received: 29 August 2012; in revised form: 31 October 2012 / Accepted: 2 November 2012 / Published: 13 November 2012
(This article belongs to the Special Issue Supported Lipid Membranes)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Models for the organization of sterols into regular arrays within phospholipid bilayers have been proposed previously. The existence of such arrays in real systems has been supported by the fact that concentration-dependent sterol properties show discontinuities at the cholesterol mole fractions corresponding to regular lattice arrangements. Experimental results presented here are based on a surface plasmon resonance assay that was used to analyze rates of cyclodextrin-mediated removal of cholesterol from adsorbed liposomes at cholesterol mole fractions up to χC = 0.55. Two kinetic pools of cholesterol were detected; there was a fast pool present at χC > 0.25, and a slow pool, with a removal rate that was dependent on the initial χC but that did not vary as χC decreased during the course of one experiment. The cholesterol activity therefore seems to be affected by sample history as well as local concentration, which could be explained in terms of the formation of superlattices that are stable for relatively long times. We also describe a variation on the traditional lattice models, with phosphatidylcholine (PC) being treated as an arrangement of hexagonal tiles; the cholesterol is then introduced at any vertex point, without increasing the total area occupied by all the lipid molecules. This model is consistent with Langmuir trough measurements of total lipid area and provides a simple explanation for the maximum solubility of cholesterol in the PC bilayer.
Keywords: cholesterol; cyclodextrin; liposome; membrane; membrane lateral organisation; OPPC; POPC; solubility; superlattice; surface plasmon resonance
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MDPI and ACS Style

Melzak, K.A.; Melzak, S.A.; Gizeli, E.; Toca-Herrera, J.L. Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study. Materials 2012, 5, 2306-2325.

AMA Style

Melzak KA, Melzak SA, Gizeli E, Toca-Herrera JL. Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study. Materials. 2012; 5(11):2306-2325.

Chicago/Turabian Style

Melzak, Kathryn A.; Melzak, Shirley A.; Gizeli, Electra; Toca-Herrera, José L. 2012. "Cholesterol Organization in Phosphatidylcholine Liposomes: A Surface Plasmon Resonance Study." Materials 5, no. 11: 2306-2325.

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