Materials 2012, 5(12), 2621-2636; doi:10.3390/ma5122621
Polydopamine-Supported Lipid Bilayers
1
Department of Chemistry, Université du Québec à Montréal, C.P. 8888, Succursale Centre-Ville, Montréal, Québec H3C 3P8, Canada
2
Department of Chemistry, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, Québec H3C 3J7, Canada
3
Faculty of Pharmacy, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, Québec H3C 3J7, Canada
*
Authors to whom correspondence should be addressed.
Received: 8 October 2012 / Revised: 27 November 2012 / Accepted: 29 November 2012 / Published: 4 December 2012
(This article belongs to the Special Issue Supported Lipid Membranes)
Abstract
We report the formation of lipid membranes supported by a soft polymeric cushion of polydopamine. First, 20 nm thick polydopamine films were formed on mica substrates. Atomic force microscopy imaging indicated that these films were also soft with a surface roughness of 2 nm under hydrated conditions. A zwitterionic phospholipid bilayer was then deposited on the polydopamine cushion by fusion of dimyristoylphosphatidylcholine (DMPC) and dioleoylphosphatidylcholine (DOPC) vesicles. Polydopamine films preserved the lateral mobility of the phospholipids as shown by fluorescence microscopy recovery after photobleaching (FRAP) experiments. Diffusion coefficients of ~5.9 and 7.2 µm2 s−1 were respectively determined for DMPC and DOPC at room temperature, values which are characteristic of lipids in a free standing bilayer system. View Full-Text
Figure 1
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MDPI and ACS Style
Nirasay, S.; Badia, A.; Leclair, G.; Claverie, J.P.; Marcotte, I. Polydopamine-Supported Lipid Bilayers. Materials 2012, 5, 2621-2636.
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