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Materials 2017, 10(11), 1303; doi:10.3390/ma10111303

Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery

1
Institute for Biologically Inspired Materials, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Vienna, Austria
2
Institute for Synthetic Bioarchitectures, Department of Nanobiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Vienna, Austria
*
Author to whom correspondence should be addressed.
Received: 18 October 2017 / Revised: 7 November 2017 / Accepted: 10 November 2017 / Published: 13 November 2017
(This article belongs to the Special Issue Polymeric Materials for Medical Applications)
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Abstract

We present a potential theranostic delivery platform based on the amphiphilic diblock copolymer polybutadiene-block-poly (ethylene oxide) combining covalent fluorescent labeling and membrane incorporation of superparamagnetic iron oxide nanoparticles for multimodal imaging. A simple self-assembly and labeling approach to create the fluorescent and magnetic vesicles is described. Cell uptake of the densely PEGylated polymer vesicles could be altered by surface modifications that vary surface charge and accessibility of the membrane active species. Cell uptake and cytotoxicity were evaluated by confocal microscopy, transmission electron microscopy, iron content and metabolic assays, utilizing multimodal tracking of membrane fluorophores and nanoparticles. Cationic functionalization of vesicles promoted endocytotic uptake. In particular, incorporation of cationic lipids in the polymersome membrane yielded tremendously increased uptake of polymersomes and magnetopolymersomes without increase in cytotoxicity. Ultrastructure investigations showed that cationic magnetopolymersomes disintegrated upon hydrolysis, including the dissolution of incorporated iron oxide nanoparticles. The presented platform could find future use in theranostic multimodal imaging in vivo and magnetically triggered delivery by incorporation of thermorepsonsive amphiphiles that can break the membrane integrity upon magnetic heating via the embedded superparamagnetic nanoparticles. View Full-Text
Keywords: superparamagnetic iron oxide nanoparticle; cationic magnetopolymersomes; cationic lipopolymersomes; confocal microscopy; transmission electron microscopy; cell-uptake; cytotoxicity superparamagnetic iron oxide nanoparticle; cationic magnetopolymersomes; cationic lipopolymersomes; confocal microscopy; transmission electron microscopy; cell-uptake; cytotoxicity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bixner, O.; Gal, N.; Zaba, C.; Scheberl, A.; Reimhult, E. Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery. Materials 2017, 10, 1303.

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