Next Article in Journal
Experimental and Computational Study of Ductile Fracture in Small Punch Tests
Next Article in Special Issue
Fluorescent Magnetopolymersomes: A Theranostic Platform to Track Intracellular Delivery
Previous Article in Journal
Bioactivity and Cell Compatibility of β-Wollastonite Derived from Rice Husk Ash and Limestone
Previous Article in Special Issue
Surface Modification of Polymer Substrates for Biomedical Applications
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Materials 2017, 10(10), 1177; https://doi.org/10.3390/ma10101177

Development of Useful Biomaterial for Bone Tissue Engineering by Incorporating Nano-Copper-Zinc Alloy (nCuZn) in Chitosan/Gelatin/Nano-Hydroxyapatite (Ch/G/nHAp) Scaffold

1
Programa de Doctorado en Biotecnología, Pontificia Universidad Católica de Valparaíso/Universidad Técnica Federico Santa María, Valparaíso 2340000, Chile
2
Instituto de Química, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso 2340000, Chile
3
Centro de Biotecnología and Departamento de Física, Universidad Técnica Federico Santa María, Valparaíso 2340000, Chile
*
Author to whom correspondence should be addressed.
Received: 28 July 2017 / Revised: 29 September 2017 / Accepted: 10 October 2017 / Published: 17 October 2017
(This article belongs to the Special Issue Polymeric Materials for Medical Applications)
Full-Text   |   PDF [3390 KB, uploaded 18 October 2017]   |  

Abstract

Ceramic and metallic nanoparticles can improve the mechanical and biological properties of polymeric scaffolds for bone tissue engineering (BTE). In this work, nanohydroxyapatite (nHAp) and nano-copper-zinc alloy (nCuZn) were added to a chitosan/gelatin (Ch/G) scaffold in order to investigate the effects on morphological, physical, and biocompatibility properties. Scaffolds were fabricated by a freeze-drying technique using different pre-freezing temperatures. Microstructure and morphology were studied by scanning electron microscopy (SEM), glass transition (Tg) was studied using differential scanning calorimetry (DSC), cell growth was estimated by MTT assay, and biocompatibility was examined in vitro and in vivo by histochemistry analyses. Scaffolds and nanocomposite scaffolds presented interconnected pores, high porosity, and pore size appropriate for BTE. Tg of Ch/G scaffolds was diminished by nanoparticle inclusion. Mouse embryonic fibroblasts (MEFs) cells loaded in the Ch/G/nHAp/nCuZn nanocomposite scaffold showed suitable behavior, based on cell adhesion, cell growth, alkaline phosphatase (ALP) activity as a marker of osteogenic differentiation, and histological in vitro cross sections. In vivo subcutaneous implant showed granulation tissue formation and new tissue infiltration into the scaffold. The favorable microstructure, coupled with the ability to integrate nanoparticles into the scaffold by freeze-drying technique and the biocompatibility, indicates the potential of this new material for applications in BTE. View Full-Text
Keywords: scaffold; metallic nanoparticles; chitosan; gelatin; nanohydroxyapatite; bone tissue engineering scaffold; metallic nanoparticles; chitosan; gelatin; nanohydroxyapatite; bone tissue engineering
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Forero, J.C.; Roa, E.; Reyes, J.G.; Acevedo, C.; Osses, N. Development of Useful Biomaterial for Bone Tissue Engineering by Incorporating Nano-Copper-Zinc Alloy (nCuZn) in Chitosan/Gelatin/Nano-Hydroxyapatite (Ch/G/nHAp) Scaffold. Materials 2017, 10, 1177.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top