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Simultaneous Isolation of Lactoferrin and Lactoperoxidase from Bovine Colostrum by SPEC 70 SLS Cation Exchange Resin
Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang Province, 310027, China
People Hospital of Jinhua City, Jinhua, Zhejiang Province, 321000, China
Zhejiang Academy of Traditional Chinese Medicine, Hangzhou, Zhejiang Province, 310007, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 19 July 2011; in revised form: 19 August 2011 / Accepted: 31 August 2011 / Published: 21 September 2011
Abstract: In this work, simultaneous isolation of lactoferrin (Lf) and lactoperoxidase (Lp) from defatted bovine colostrum by one-step cation exchange chromatography with SPEC 70 SLS ion-exchange resin was investigated. A RP-HPLC method for Lf and Lp determination was developed and optimized as the following conditions: detection wavelength of 220 nm, flow rate of 1 mL/min and acetonitrile concentration from 25% to 75% within 20 min. The adsorption process of Lf on SPEC 70 SLS resin was optimized using Lf standard as substrate. The maximum static binding capacity of SPEC 70 SLS resin was of 22.0 mg/g resin at 15 °С, pH 7.0 and adsorption time 3 h. The Lf adsorption process could be well described by the Langmuir adsorption isotherm model, with a maximum adsorption capacity of 21.73 mg/g resin at 15 °С. In batch fractionation of defatted colostrum, the binding capacities of SPEC 70 SLS resin for adsorbing Lf and Lp simultaneously under the abovementioned conditions were 7.60 and 6.89 mg/g resin, respectively, both of which were superior to those of CM Sepharose F.F. or SP Sepharose F.F. resins under the same conditions. As a result, SPEC 70 SLS resin was considered as a successful candidate for direct and economic purification of Lf and Lp from defatted colostrum.
Keywords: defatted colostrum; lactoferrin; lactoperoxidase; SPEC 70 SLS resin; isolation
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MDPI and ACS Style
Liang, Y.; Wang, X.; Wu, M.; Zhu, W. Simultaneous Isolation of Lactoferrin and Lactoperoxidase from Bovine Colostrum by SPEC 70 SLS Cation Exchange Resin. Int. J. Environ. Res. Public Health 2011, 8, 3764-3776.
Liang Y, Wang X, Wu M, Zhu W. Simultaneous Isolation of Lactoferrin and Lactoperoxidase from Bovine Colostrum by SPEC 70 SLS Cation Exchange Resin. International Journal of Environmental Research and Public Health. 2011; 8(9):3764-3776.
Liang, Yafei; Wang, Xuewan; Wu, Mianbin; Zhu, Wanping. 2011. "Simultaneous Isolation of Lactoferrin and Lactoperoxidase from Bovine Colostrum by SPEC 70 SLS Cation Exchange Resin." Int. J. Environ. Res. Public Health 8, no. 9: 3764-3776.