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Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage
Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway
NOFIMA Mat, Ås, Norway
NIVA Norwegian Water Research Institute, Oslo, Norway
* Author to whom correspondence should be addressed.
Received: 20 July 2010; in revised form: 3 August 2010 / Accepted: 25 August 2010 / Published: 31 August 2010
Abstract: We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.
Keywords: viable/dead cells; UV; quantitative PCR
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MDPI and ACS Style
Rudi, K.; Hagen, I.; Johnsrud, B.C.; Skjefstad, G.; Tryland, I. Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage. Int. J. Environ. Res. Public Health 2010, 7, 3376-3381.
Rudi K, Hagen I, Johnsrud BC, Skjefstad G, Tryland I. Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage. International Journal of Environmental Research and Public Health. 2010; 7(9):3376-3381.
Rudi, Knut; Hagen, Irina; Johnsrud, Bente Carina; Skjefstad, Guro; Tryland, Ingun. 2010. "Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage." Int. J. Environ. Res. Public Health 7, no. 9: 3376-3381.