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Int. J. Environ. Res. Public Health 2010, 7(9), 3376-3381; doi:10.3390/ijerph7093376

Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage

1
Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway
2
NOFIMA Mat, Ås, Norway
3
NIVA Norwegian Water Research Institute, Oslo, Norway
*
Author to whom correspondence should be addressed.
Received: 20 July 2010 / Revised: 3 August 2010 / Accepted: 25 August 2010 / Published: 31 August 2010
(This article belongs to the Special Issue Drinking Water and Health)
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Abstract

We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing. View Full-Text
Keywords: viable/dead cells; UV; quantitative PCR viable/dead cells; UV; quantitative PCR
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Rudi, K.; Hagen, I.; Johnsrud, B.C.; Skjefstad, G.; Tryland, I. Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage. Int. J. Environ. Res. Public Health 2010, 7, 3376-3381.

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