Abstract: The objective of this study was to evaluate the reproductive risk associated with exposure of adult male Fisher-344 (F-344) rats to inhaled benzo(a)pyrene (BaP), a ubiquitous environmental toxicant present in cigarette smoke, automobile exhaust fumes and industrial emissions. Rats were assigned randomly to a treatment or control group. Treatment consisted of exposure of rats via nose-only inhalation to 75μg BaP/m3, 4 hours daily for 60 days, while control animals were unexposed (UNC). Blood samples were collected immediately on day 60 of exposures (time 0) and subsequently at 24, 48, and 72 hours, to assess the effect of exposures to BaP on plasma testosterone and luteinizing hormone (LH) concentrations. Mean testis weight, total weight of tubules and total tubular length per paired testes were reduced 33% (P< 0.025), 27% (P < 0.01) and 39%, respectively in exposed rats (P < 0.01) compared with UNC rats. The number of homogenization -resistant spermatids was significantly reduced in BaP-exposed versus UNC rats. Plasma testosterone and intra-testicular testosterone (ITT) concentrations were significantly decreased by BaP compared with those of UNC rats. The decreases in circulating plasma testosterone were accompanied by concomitant increases in plasma LH concentrations in BaP-exposed versus control rats (P < 0.05). These data suggest that 60 days exposure to inhaled BaP contribute to reduced testicular endocrine and spermatogenic functions in exposed rats.
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Archibong, A.E.; Ramesh, A.; Niaz, M.S.; Brooks, C.M.; Roberson, S.I.; Lunstra, D.D. Effects of Benzo(a)pyrene on Intra-testicular Function in F-344 Rats. Int. J. Environ. Res. Public Health 2008, 5, 32-40.
Archibong AE, Ramesh A, Niaz MS, Brooks CM, Roberson SI, Lunstra DD. Effects of Benzo(a)pyrene on Intra-testicular Function in F-344 Rats. International Journal of Environmental Research and Public Health. 2008; 5(1):32-40.
Archibong, Anthony E.; Ramesh, Aramandla; Niaz, Mohammad S.; Brooks, Cynthia M.; Roberson, Shannon I.; Lunstra, Donald D. 2008. "Effects of Benzo(a)pyrene on Intra-testicular Function in F-344 Rats." Int. J. Environ. Res. Public Health 5, no. 1: 32-40.