The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China)
AbstractAntibiotic failure is occurring worldwide. In a routine surveillance study on antibioticresistance genes (ARGs) in natural water bodies, we noted the detection of colistin-resistance gene mcr-1, previously identified in Escherichia coli and Klebsiella pneumoniae isolates from human beings and animals in several countries. The mcr-1 gene might be present in water environments, because aquatic ecosystems are recognized as reservoirs for antibiotic resistant bacteria (ARB) and ARGs. In this study, a qPCR assay was developed to monitor and quantify the mcr-1 gene in the Haihe River, China. The results showed that all 18 samples collected from different locations over 6 months along the Haihe River were positive for the mcr-1 gene, and the highest level of mcr-1 reached 3.81 × 105 gene copies (GC) per liter of water. This is the first study to quantify mcr-1 in a natural water system by qPCR. Our findings highlight the potential for this antibiotic resistance determinant to spread extensively, suggesting a significant health and ecological impact. View Full-Text
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Yang, D.; Qiu, Z.; Shen, Z.; Zhao, H.; Jin, M.; Li, H.; Liu, W.; Li, J.-W. The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China). Int. J. Environ. Res. Public Health 2017, 14, 576.
Yang D, Qiu Z, Shen Z, Zhao H, Jin M, Li H, Liu W, Li J-W. The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China). International Journal of Environmental Research and Public Health. 2017; 14(6):576.Chicago/Turabian Style
Yang, Dong; Qiu, Zhigang; Shen, Zhiqiang; Zhao, Hong; Jin, Min; Li, Huaying; Liu, Weili; Li, Jun-Wen. 2017. "The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China)." Int. J. Environ. Res. Public Health 14, no. 6: 576.
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