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Int. J. Environ. Res. Public Health 2017, 14(11), 1400; doi:10.3390/ijerph14111400

Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry

1
Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
2
Risk Analysis Research Center, Sookmyung Women’s University, Seoul 04310, Korea
3
Department of Veterinary Medicine, Konkuk University, Seoul 05029, Korea
*
Authors to whom correspondence should be addressed.
Received: 27 September 2017 / Revised: 8 November 2017 / Accepted: 14 November 2017 / Published: 17 November 2017
(This article belongs to the Special Issue Antimicrobials and Antimicrobial Resistance in the Environment)
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Abstract

This study examined antibiotic susceptibility, genetic diversity, and characteristics of virulence genes in Campylobacter isolates from poultry. Chicken (n = 152) and duck (n = 154) samples were collected from 18 wet markets in Korea. Campylobacter spp. isolated from the carcasses were identified by PCR. The isolated colonies were analyzed for antibiotic susceptibility to chloramphenicol, amikacin, erythromycin, tetracycline, ciprofloxacin, nalidixic acid, and enrofloxacin. The isolates were also used to analyze genetic diversity using the DiversiLabTM system and were tested for the presence of cytolethal distending toxin (cdt) genes. Campylobacter spp. were isolated from 45 poultry samples out of 306 poultry samples (14.7%) and the average levels of Campylobacter contamination were 22.0 CFU/g and 366.1 CFU/g in chicken and duck samples, respectively. Moreover, more than 90% of the isolates showed resistance to nalidixic acid and ciprofloxacin. Genetic correlation analysis showed greater than 95% similarity between 84.4% of the isolates, and three cdt genes (cdtA, cdtB, and cdtC) were present in 71.1% of Campylobacter isolates. These results indicate that Campylobacter contamination should be decreased to prevent and treat Campylobacter foodborne illness. View Full-Text
Keywords: Campylobacter; poultry; antibiotic susceptibility; Rep-PCR; cdt toxin Campylobacter; poultry; antibiotic susceptibility; Rep-PCR; cdt toxin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lee, J.; Jeong, J.; Lee, H.; Ha, J.; Kim, S.; Choi, Y.; Oh, H.; Seo, K.; Yoon, Y.; Lee, S. Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry. Int. J. Environ. Res. Public Health 2017, 14, 1400.

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