Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Measurement and Collection of Samples
2.3. Immunohistochemistry
2.4. Immunohistochemical Evaluation
2.5. Statistical Analysis
3. Results
3.1. Sample Clinical Characteristics
3.2. Maternal and Cord Blood Iron Metabolism Biochemistry
3.3. Expression of FPN, Hep, and TfR1 in the Placenta
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
GDM | Gestational Diabetes |
STfR | Soluble transferrin receptor (sTfR) |
SF | Serum ferritin |
FPN | Ferroportin |
NGT | Normal glucose tolerance |
STB | Syncytiotrophoblast |
HCT | Hematocrit |
OGTT | Oral glucose tolerance test |
HEP | Hepcidin |
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Clinical Features | GDM (n = 42) | Non GDM (n = 40) | p Value |
---|---|---|---|
Maternal age (year) | 30.52 ± 3.81 | 29.39 ± 3.76 | 0.090 |
BMI * | 27.10 ± 2.39 | 25.96 ± 2.07 | 0.032 |
Newborn birth weight (kg) | 3.25 ± 0.39 | 3.10 ± 0.34 | 0.083 |
Wk of gestation (week) | 38.71 ± 1.0 | 37.70 ± 2.86 | 0.071 |
OGTT fasting (mg/dL) | |||
1-h * | 10.30 ± 1.11 | 8.92 ± 0.94 | 0.000 |
2-h * | 9.21 ± 1.22 | 7.71 ± 0.69 | 0.000 |
Group | GDM (n = 42) | Control (n = 40) | p Value |
---|---|---|---|
Maternal Hb (g/dL) | 116.20 ± 7.0 | 117.76 ± 8.31 | 0.391 |
Umbilical cord Hb (g/dL) | 158.14 ± 9.54 | 154.76 ± 11.51 | 0.182 |
Maternal blood sTfR (nM) | 31.82 ± 11.51 | 22.80 ± 8.30 | 0.003 a |
Cord blood sTfR (nM) | 34.63 ± 8.33 | 32.60 ± 6.00 | 0.364 |
Maternal SF (ng/mL) | 21.71 (13.86–42.62) Δ | 30.90 (25.00–39.00) Δ | 0.118 |
Umbilical cord SF (ng/mL) | 172.75 (112.08–192.03) Δ | 111.6 (68.29–150.95) Δ | 0.003 b |
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Yang, A.; Zhao, J.; Lu, M.; Gu, Y.; Zhu, Y.; Chen, D.; Fu, J. Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes. Int. J. Environ. Res. Public Health 2016, 13, 766. https://doi.org/10.3390/ijerph13080766
Yang A, Zhao J, Lu M, Gu Y, Zhu Y, Chen D, Fu J. Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes. International Journal of Environmental Research and Public Health. 2016; 13(8):766. https://doi.org/10.3390/ijerph13080766
Chicago/Turabian StyleYang, Anqiang, Jun Zhao, Minhua Lu, Ying Gu, Yunlong Zhu, Daozhen Chen, and Jinyan Fu. 2016. "Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes" International Journal of Environmental Research and Public Health 13, no. 8: 766. https://doi.org/10.3390/ijerph13080766