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Int. J. Environ. Res. Public Health 2016, 13(5), 483; doi:10.3390/ijerph13050483

Effects of Size-Fractionated Particulate Matter on Cellular Oxidant Radical Generation in Human Bronchial Epithelial BEAS-2B Cells

Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
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Academic Editor: Paul B. Tchounwou
Received: 22 March 2016 / Revised: 19 April 2016 / Accepted: 26 April 2016 / Published: 10 May 2016
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Abstract

The aim of the present study was to investigate the effects of size-fractionated (i.e., <1; 1–2.5, and 2.5–10 µm in an aerodynamic diameter) ambient particulate matter (PM) on reactive oxygen species (ROS) activity and cell viability in human bronchial epithelial cells (BEAS-2B). The PM samples were collected from an urban site (uPM) in Beijing and a steel factory site (sPM) in Anshan, China, from March 2013 to December 2014. Metal elements, organic and elemental carbon, and water-soluble inorganic ions in the uPM and sPM were analyzed. The cell viability and ROS generation in PM-exposed BEAS-2B cells were measured by MTS and DCFH-DA. The results showed that both uPM and sPM caused a decrease in the cell viability and an increase in ROS generation. The level of ROS measured in sPM1.0 was approximately triple that in uPM1.0. The results of correlation analysis showed that the ROS activity and cytotoxicity were related to different PM composition. Moreover, deferoxamine (DFO) significantly prevented the increase of ROS generation and the decrease of cell viability. Taken together, our results suggest that the metals absorbed on PM induced oxidant radical generation in BEAS-2B cells that could lead to impairment of pulmonary function. View Full-Text
Keywords: particulate matter; size-fraction; chemical composition; correlation analysis; cytotoxicity particulate matter; size-fraction; chemical composition; correlation analysis; cytotoxicity
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MDPI and ACS Style

Guan, L.; Rui, W.; Bai, R.; Zhang, W.; Zhang, F.; Ding, W. Effects of Size-Fractionated Particulate Matter on Cellular Oxidant Radical Generation in Human Bronchial Epithelial BEAS-2B Cells. Int. J. Environ. Res. Public Health 2016, 13, 483.

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