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Reply published on 6 January 2016, see Int. J. Environ. Res. Public Health 2016, 13(1), 109.

Comment of Int. J. Environ. Res. Public Health 2015, 12(10), 12466-12474.

Open AccessComment
Int. J. Environ. Res. Public Health 2016, 13(1), 108; doi:10.3390/ijerph13010108

Comments on Scheffler et al. Cytotoxic Evaluation of E-Liquid Aerosol using Different Lung Derived Cell Models. Int. J. Environ. Res. Public Health, 2015, 12, 12466-12474

1
Dipartimento di Medicina Clinica e Sperimentale, Università di Catania, Via Palermo 636, 95121 Catania, Italy
2
Dipartimento di Scienze Biomediche e Biotecnologiche, V.le A. Doria 6, 95125 Catania, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 24 December 2015 / Accepted: 31 December 2015 / Published: 6 January 2016
View Full-Text   |   Download PDF [227 KB, uploaded 6 January 2016]

Abstract

There is merit in considering a simple toxicological screening method that evaluates the total cytotoxic potential of e-liquids or electronic cigarettes (ECs) aerosol emissions in one single testing. However, there is growing confusion, with several researchers endorsing their personal solution to the problem. Here, we discuss as an example the recent paper by Scheffler and colleagues, in which the authors suggest that more relevant and well differentiated cell lines from human airways could be the most suitable candidates for toxicological evaluation of ECs aerosol emissions. We advance recommendations for validated protocols and advocate for an international coordinated effort aimed at establishing consensus on methodology. View Full-Text
Keywords: vapour emission; electronic cigarette; cytotoxicity; tobacco product directive vapour emission; electronic cigarette; cytotoxicity; tobacco product directive
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Polosa, R.; Caruso, M.; Guarino, F. Comments on Scheffler et al. Cytotoxic Evaluation of E-Liquid Aerosol using Different Lung Derived Cell Models. Int. J. Environ. Res. Public Health, 2015, 12, 12466-12474. Int. J. Environ. Res. Public Health 2016, 13, 108.

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