Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models
AbstractThe in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX® RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells. View Full-Text
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Scheffler, S.; Dieken, H.; Krischenowski, O.; Aufderheide, M. Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models. Int. J. Environ. Res. Public Health 2015, 12, 12466-12474.
Scheffler S, Dieken H, Krischenowski O, Aufderheide M. Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models. International Journal of Environmental Research and Public Health. 2015; 12(10):12466-12474.Chicago/Turabian Style
Scheffler, Stefanie; Dieken, Hauke; Krischenowski, Olaf; Aufderheide, Michaela. 2015. "Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models." Int. J. Environ. Res. Public Health 12, no. 10: 12466-12474.