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Comment published on 6 January 2016, see Int. J. Environ. Res. Public Health 2016, 13(1), 108.

Open AccessArticle
Int. J. Environ. Res. Public Health 2015, 12(10), 12466-12474; doi:10.3390/ijerph121012466

Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models

Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany
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Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 14 July 2015 / Revised: 28 September 2015 / Accepted: 30 September 2015 / Published: 5 October 2015
View Full-Text   |   Download PDF [306 KB, uploaded 5 October 2015]   |  

Abstract

The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX® RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells. View Full-Text
Keywords: electronic cigarette; smoking; tobacco; nicotine; cytotoxicity; immortalized normal bronchial epithelial cells; air-liquid interface; CL-1548; CULTEX® RFS; public health electronic cigarette; smoking; tobacco; nicotine; cytotoxicity; immortalized normal bronchial epithelial cells; air-liquid interface; CL-1548; CULTEX® RFS; public health
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Scheffler, S.; Dieken, H.; Krischenowski, O.; Aufderheide, M. Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models. Int. J. Environ. Res. Public Health 2015, 12, 12466-12474.

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