The AhR Ligand, TCDD, Regulates Androgen Receptor Activity Differently in Androgen-Sensitive versus Castration-Resistant Human Prostate Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. Protein Isolation and Western Blot Analysis
2.4. Immunocytochemical Staining and Fluorescence Microscopy
2.5. RNA Extraction and Quantitative RT-PCR Analysis
2.6. Statistical Analysis
3. Results
3.1. TCDDs Effect on AhR and AR Protein Expression in LNCaP and C4-2 Prostate Cancer Cells
3.2. TCDD Induces Nuclear Localization of AR in LNCaP but Not in C4-2 Prostate Cancer Cells
3.3. TCDD Enhances Expression of Androgen Responsive Gene KLK3 in LNCaP Cells
3.4. TCDD Induces AR Phosphorylation in LNCaP Cells
4. Discussion
5. Conclusions
Acknowledgements
Author Contributions
Conflicts of Interest
References
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Ghotbaddini, M.; Powell, J.B. The AhR Ligand, TCDD, Regulates Androgen Receptor Activity Differently in Androgen-Sensitive versus Castration-Resistant Human Prostate Cancer Cells. Int. J. Environ. Res. Public Health 2015, 12, 7506-7518. https://doi.org/10.3390/ijerph120707506
Ghotbaddini M, Powell JB. The AhR Ligand, TCDD, Regulates Androgen Receptor Activity Differently in Androgen-Sensitive versus Castration-Resistant Human Prostate Cancer Cells. International Journal of Environmental Research and Public Health. 2015; 12(7):7506-7518. https://doi.org/10.3390/ijerph120707506
Chicago/Turabian StyleGhotbaddini, Maryam, and Joann B. Powell. 2015. "The AhR Ligand, TCDD, Regulates Androgen Receptor Activity Differently in Androgen-Sensitive versus Castration-Resistant Human Prostate Cancer Cells" International Journal of Environmental Research and Public Health 12, no. 7: 7506-7518. https://doi.org/10.3390/ijerph120707506