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Int. J. Environ. Res. Public Health 2015, 12(5), 5386-5405; doi:10.3390/ijerph120505386

Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes

1
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, E-38040 Madrid, Spain
2
Faculty of Vetsuisse, Centre for Fish and Wildlife Health, University of Berne, Länggassstr. 122, Postfach 8466, CH-3001 Bern, Switzerland,
*
Author to whom correspondence should be addressed.
Academic Editor: Mónica Amorim
Received: 26 March 2015 / Revised: 30 April 2015 / Accepted: 12 May 2015 / Published: 20 May 2015
(This article belongs to the Special Issue Environmental Fate and Effect of Nanoparticles and Nanomaterials)
View Full-Text   |   Download PDF [1668 KB, uploaded 20 May 2015]   |  

Abstract

Among all classes of nanomaterials, silver nanoparticles (AgNPs) have potentially an important ecotoxicological impact, especially in freshwater environments. Fish are particularly susceptible to the toxic effects of silver ions and, with knowledge gaps regarding the contribution of dissolution and unique particle effects to AgNP toxicity, they represent a group of vulnerable organisms. Using cell lines (RTL-W1, RTH-149, RTG-2) and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) as in vitro test systems, we assessed the cytotoxicity of the representative AgNP, NM-300K, and AgNO3 as an Ag+ ion source. Lack of AgNP interference with the cytotoxicity assays (AlamarBlue, CFDA-AM, NRU assay) and their simultaneous application point to the compatibility and usefulness of such a battery of assays. The RTH-149 and RTL-W1 liver cell lines exhibited similar sensitivity as primary hepatocytes towards AgNP toxicity. Leibovitz’s L-15 culture medium composition (high amino acid content) had an important influence on the behaviour and toxicity of AgNPs towards the RTL-W1 cell line. The obtained results demonstrate that, with careful consideration, such an in vitro approach can provide valuable toxicological data to be used in an integrated testing strategy for NM-300K risk assessment. View Full-Text
Keywords: AgNPs; cytotoxicity; rainbow trout (Oncorhynchus mykiss); in vitro cell lines; primary hepatocyte AgNPs; cytotoxicity; rainbow trout (Oncorhynchus mykiss); in vitro cell lines; primary hepatocyte
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Connolly, M.; Fernandez-Cruz, M.-L.; Quesada-Garcia, A.; Alte, L.; Segner, H.; Navas, J.M. Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes. Int. J. Environ. Res. Public Health 2015, 12, 5386-5405.

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