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Int. J. Environ. Res. Public Health 2014, 11(8), 8581-8596; doi:10.3390/ijerph110808581
Article

Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles

1,†
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Received: 30 June 2014; in revised form: 4 August 2014 / Accepted: 12 August 2014 / Published: 20 August 2014
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Abstract: Nine types of solid lipid nanoparticle (SLN) formulations were produced using tripalmitin (TPM), glyceryl monostearate (GM) or stearic acid (SA), stabilized with lecithin S75 and polysorbate 80. Formulations were prepared presenting PI values within 0.25 to 0.30, and the physicochemical properties, stability upon storage and biocompatibility were evaluated. The average particle size ranged from 116 to 306 nm, with a negative surface charge around −11 mV. SLN presented good stability up to 60 days. The SLN manufactured using SA could not be measured by DLS due to the reflective feature of this formulation. However, TEM images revealed that SA nanoparticles presented square/rod shapes with an approximate size of 100 nm. Regarding biocompatibility aspects, SA nanoparticles showed toxicity in fibroblasts, causing cell death, and produced high hemolytic rates, indicating toxicity to red blood cells. This finding might be related to lipid type, as well as, the shape of the nanoparticles. No morphological alterations and hemolytic effects were observed in cells incubated with SLN containing TPM and GM. The SLN containing TPM and GM showed long-term stability, suggesting good shelf-life. The results indicate high toxicity of SLN prepared with SA, and strongly suggest that the components of the formulation should be analyzed in combination rather than separately to avoid misinterpretation of the results.
Keywords: solid lipid nanoparticles; cytotoxicity; surfactant; lipid; biocompatibility solid lipid nanoparticles; cytotoxicity; surfactant; lipid; biocompatibility
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pizzol, C.D.; Filippin-Monteiro, F.B.; Restrepo, J.A.S.; Pittella, F.; Silva, A.H.; Alves de Souza, P.; Machado de Campos, A.; Creczynski-Pasa, T.B. Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles. Int. J. Environ. Res. Public Health 2014, 11, 8581-8596.

AMA Style

Pizzol CD, Filippin-Monteiro FB, Restrepo JAS, Pittella F, Silva AH, Alves de Souza P, Machado de Campos A, Creczynski-Pasa TB. Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles. International Journal of Environmental Research and Public Health. 2014; 11(8):8581-8596.

Chicago/Turabian Style

Pizzol, Carine D.; Filippin-Monteiro, Fabíola B.; Restrepo, Jelver A.S.; Pittella, Frederico; Silva, Adny H.; Alves de Souza, Paula; Machado de Campos, Angela; Creczynski-Pasa, Tânia B. 2014. "Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles." Int. J. Environ. Res. Public Health 11, no. 8: 8581-8596.


Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert