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Int. J. Environ. Res. Public Health 2014, 11(6), 5708-5719; doi:10.3390/ijerph110605708
Article

Evaluation of the Relationship between the Adenosine Triphosphate (ATP) Bioluminescence Assay and the Presence of Bacillus anthracis Spores and Vegetative Cells

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Received: 8 April 2014; in revised form: 15 May 2014 / Accepted: 20 May 2014 / Published: 28 May 2014
(This article belongs to the Special Issue Environmental Determinants of Infectious Disease Transmission)
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Abstract: Background: The Adenosine triphosphate (ATP) bioluminescence assay was utilized in laboratory evaluations to determine the presence and concentration of vegetative and spore forms of Bacillus anthracis Sterne 34F2. Methods: Seventeen surfaces from the healthcare environment were selected for evaluation. Surfaces were inoculated with 50 µL of organism suspensions at three concentrations of 104, 106, 108 colony forming units per surface (CFU/surface) of B. anthracis. Culture-based methods and ATP based methods were utilized to determine concentrations. Results: When all concentrations were evaluated together, a positive correlation between log-adjusted CFU and Relative Light Units (RLU) for endospores and vegetative cells was established. When concentrations were evaluated separately, a significant correlation was not demonstrated. Conclusions: This study demonstrated a positive correlation for ATP and culture-based methods for the vegetative cells of B. anthracis. When evaluating the endospores and combining both metabolic states, the ATP measurements and CFU recovered did not correspond to the initial concentrations on the evaluated surfaces. The results of our study show that the low ATP signal which does not correlate well to the CFU results would not make the ATP measuring devises effective in confirming contamination residual from a bioterrorist event.
Keywords: ATP measurement; Bacillus anthracis; cleaning confirmation; rapid detection ATP measurement; Bacillus anthracis; cleaning confirmation; rapid detection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Gibbs, S.G.; Sayles, H.; Colbert, E.M.; Hewlett, A.; Chaika, O.; Smith, P.W. Evaluation of the Relationship between the Adenosine Triphosphate (ATP) Bioluminescence Assay and the Presence of Bacillus anthracis Spores and Vegetative Cells. Int. J. Environ. Res. Public Health 2014, 11, 5708-5719.

AMA Style

Gibbs SG, Sayles H, Colbert EM, Hewlett A, Chaika O, Smith PW. Evaluation of the Relationship between the Adenosine Triphosphate (ATP) Bioluminescence Assay and the Presence of Bacillus anthracis Spores and Vegetative Cells. International Journal of Environmental Research and Public Health. 2014; 11(6):5708-5719.

Chicago/Turabian Style

Gibbs, Shawn G.; Sayles, Harlan; Colbert, Erica M.; Hewlett, Angela; Chaika, Oleg; Smith, Philip W. 2014. "Evaluation of the Relationship between the Adenosine Triphosphate (ATP) Bioluminescence Assay and the Presence of Bacillus anthracis Spores and Vegetative Cells." Int. J. Environ. Res. Public Health 11, no. 6: 5708-5719.



Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert