Characterization of a Novel Serine Protease Inhibitor Gene from a Marine Metagenome
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cloning and Sequence Analysis of a Novel Serpin Gene
2.2. Overexpression and Purification of Recombinant Spi1C Protein
2.3. Functional Characterization of Recombinant Spi1C Protein
3. Experimental Section
3.1. Plasmid Vectors, Bacterium Strains, and Growth Conditions
3.2. DNA Manipulation and Protein Analysis
3.3. DNA Sequence Analysis, Database Search, and Gene Structure Characterization
3.4. Overexpression and Purification of the Recombinant Serpin Protein
3.5. Characterization of the Serpin Gene Spi1C
3.5.1. Elastase Inhibition Assay
3.5.2. α-Chymotrypsin Inhibition Assay
3.5.3. Trypsin Inhibition Assay
3.6. Nucleotide Sequence Accession Number
4. Conclusions
Acknowledgments
- Samples Availability: Available from the authors.
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Jiang, C.-J.; Hao, Z.-Y.; Zeng, R.; Shen, P.-H.; Li, J.-F.; Wu, B. Characterization of a Novel Serine Protease Inhibitor Gene from a Marine Metagenome. Mar. Drugs 2011, 9, 1487-1501. https://doi.org/10.3390/md9091487
Jiang C-J, Hao Z-Y, Zeng R, Shen P-H, Li J-F, Wu B. Characterization of a Novel Serine Protease Inhibitor Gene from a Marine Metagenome. Marine Drugs. 2011; 9(9):1487-1501. https://doi.org/10.3390/md9091487
Chicago/Turabian StyleJiang, Cheng-Jian, Zhen-Yu Hao, Rong Zeng, Pei-Hong Shen, Jun-Fang Li, and Bo Wu. 2011. "Characterization of a Novel Serine Protease Inhibitor Gene from a Marine Metagenome" Marine Drugs 9, no. 9: 1487-1501. https://doi.org/10.3390/md9091487