Next Article in Journal
Bioactive Cembranoids from the Dongsha Atoll Soft Coral Sarcophyton crassocaule
Previous Article in Journal
Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications
Mar. Drugs 2011, 9(6), 984-993; doi:10.3390/md9060984
Communication

DedA Protein Relates to Action-Mechanism of Halicyclamine A, a Marine Spongean Macrocyclic Alkaloid, as an Anti-dormant Mycobacterial Substance

1,* , 1
,
1
,
2
 and
1,*
Received: 1 April 2011 / Revised: 25 May 2011 / Accepted: 3 June 2011 / Published: 8 June 2011
View Full-Text   |   Download PDF [1389 KB, uploaded 24 February 2015]   |   Browse Figures

Abstract

A macrocyclic alkaloid, halicyclamine A, was re-discovered from an Indonesian marine sponge of Haliclona sp. 05A08 as an anti-dormant mycobacterial substance. To clarify action-mechanism of halicyclamine A, halicyclamine A-resistant strains were screened from the transformants of Mycobacterium smegmatis with the genomic DNA library of M. bovis BCG, which were constructed in the multi-copy shuttle cosmid pYUB145. Sequencing analysis of the cosmids isolated from the halicyclamine A-resistant transformants revealed that the responsible gene was involved in the genome region between 2920.549 kb and 2933.210 kb. Further experiments using the transformants over-expressing individual gene contained in the responsible region were executed, and the transformant, which over-expressed BCG2664 gene assigned as dedA gene, was found to become halicyclamine A-resistant. This evidence strongly suggested that DedA protein correlates with the action-mechanism of halicyclamine A as an anti-dormant mycobacterial substance.
Keywords: halicyclamine A; dedA; marine sponge; dormant; tuberculosis halicyclamine A; dedA; marine sponge; dormant; tuberculosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Arai, M.; Liu, L.; Fujimoto, T.; Setiawan, A.; Kobayashi, M. DedA Protein Relates to Action-Mechanism of Halicyclamine A, a Marine Spongean Macrocyclic Alkaloid, as an Anti-dormant Mycobacterial Substance. Mar. Drugs 2011, 9, 984-993.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert