Mar. Drugs 2011, 9(6), 984-993; doi:10.3390/md9060984
Communication

DedA Protein Relates to Action-Mechanism of Halicyclamine A, a Marine Spongean Macrocyclic Alkaloid, as an Anti-dormant Mycobacterial Substance

1,* email, 1email, 1email, 2email and 1,* email
Received: 1 April 2011; in revised form: 25 May 2011 / Accepted: 3 June 2011 / Published: 8 June 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A macrocyclic alkaloid, halicyclamine A, was re-discovered from an Indonesian marine sponge of Haliclona sp. 05A08 as an anti-dormant mycobacterial substance. To clarify action-mechanism of halicyclamine A, halicyclamine A-resistant strains were screened from the transformants of Mycobacterium smegmatis with the genomic DNA library of M. bovis BCG, which were constructed in the multi-copy shuttle cosmid pYUB145. Sequencing analysis of the cosmids isolated from the halicyclamine A-resistant transformants revealed that the responsible gene was involved in the genome region between 2920.549 kb and 2933.210 kb. Further experiments using the transformants over-expressing individual gene contained in the responsible region were executed, and the transformant, which over-expressed BCG2664 gene assigned as dedA gene, was found to become halicyclamine A-resistant. This evidence strongly suggested that DedA protein correlates with the action-mechanism of halicyclamine A as an anti-dormant mycobacterial substance.
Keywords: halicyclamine A; dedA; marine sponge; dormant; tuberculosis
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MDPI and ACS Style

Arai, M.; Liu, L.; Fujimoto, T.; Setiawan, A.; Kobayashi, M. DedA Protein Relates to Action-Mechanism of Halicyclamine A, a Marine Spongean Macrocyclic Alkaloid, as an Anti-dormant Mycobacterial Substance. Mar. Drugs 2011, 9, 984-993.

AMA Style

Arai M, Liu L, Fujimoto T, Setiawan A, Kobayashi M. DedA Protein Relates to Action-Mechanism of Halicyclamine A, a Marine Spongean Macrocyclic Alkaloid, as an Anti-dormant Mycobacterial Substance. Marine Drugs. 2011; 9(6):984-993.

Chicago/Turabian Style

Arai, Masayoshi; Liu, Liu; Fujimoto, Takao; Setiawan, Andi; Kobayashi, Motomasa. 2011. "DedA Protein Relates to Action-Mechanism of Halicyclamine A, a Marine Spongean Macrocyclic Alkaloid, as an Anti-dormant Mycobacterial Substance." Mar. Drugs 9, no. 6: 984-993.

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