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Mar. Drugs 2011, 9(6), 967-983; doi:10.3390/md9060967
Article

Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications

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Received: 29 April 2011; in revised form: 20 May 2011 / Accepted: 26 May 2011 / Published: 7 June 2011
(This article belongs to the Special Issue Marine Biomaterials)
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Abstract: Fibrillar collagens are the more abundant extracellular proteins. They form a metazoan-specific family, and are highly conserved from sponge to human. Their structural and physiological properties have been successfully used in the food, cosmetic, and pharmaceutical industries. On the other hand, the increase of jellyfish has led us to consider this marine animal as a natural product for food and medicine. Here, we have tested different Mediterranean jellyfish species in order to investigate the economic potential of their collagens. We have studied different methods of collagen purification (tissues and experimental procedures). The best collagen yield was obtained using Rhizostoma pulmo oral arms and the pepsin extraction method (2–10 mg collagen/g of wet tissue). Although a significant yield was obtained with Cotylorhiza tuberculata (0.45 mg/g), R. pulmo was used for further experiments, this jellyfish being considered as harmless to humans and being an abundant source of material. Then, we compared the biological properties of R. pulmo collagen with mammalian fibrillar collagens in cell cytotoxicity assays and cell adhesion. There was no statistical difference in cytotoxicity (p > 0.05) between R. pulmo collagen and rat type I collagen. However, since heparin inhibits cell adhesion to jellyfish-native collagen by 55%, the main difference is that heparan sulfate proteoglycans could be preferentially involved in fibroblast and osteoblast adhesion to jellyfish collagens. Our data confirm the broad harmlessness of jellyfish collagens, and their biological effect on human cells that are similar to that of mammalian type I collagen. Given the bioavailability of jellyfish collagen and its biological properties, this marine material is thus a good candidate for replacing bovine or human collagens in selected biomedical applications.
Keywords: collagen; jellyfish; biocompatibility; cell adhesion; cross-linking collagen; jellyfish; biocompatibility; cell adhesion; cross-linking
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Addad, S.; Exposito, J.-Y.; Faye, C.; Ricard-Blum, S.; Lethias, C. Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications. Mar. Drugs 2011, 9, 967-983.

AMA Style

Addad S, Exposito J-Y, Faye C, Ricard-Blum S, Lethias C. Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications. Marine Drugs. 2011; 9(6):967-983.

Chicago/Turabian Style

Addad, Sourour; Exposito, Jean-Yves; Faye, Clément; Ricard-Blum, Sylvie; Lethias, Claire. 2011. "Isolation, Characterization and Biological Evaluation of Jellyfish Collagen for Use in Biomedical Applications." Mar. Drugs 9, no. 6: 967-983.



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