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Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101, P.R.China
National Center for Drug Screening, Shanghai Institute of Materia Medica, Shanghai 201203, China
Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, P.R. China
University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100081, P.R. China
* Author to whom correspondence should be addressed.
Received: 11 December 2008; in revised form: 12 January 2009 / Accepted: 21 January 2009 / Published: 3 January 2009
Abstract: The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3% inhibited protein tyrosine phosphatase 1B (PTP1B), a protein related to diabetes. In addition, nine isolates inhibited aurora kinase A, an anti-cancer related protein, and three inhibited caspase 3, a protein related to neurodegenerative diseases. Representative bioactive isolates were characterized using genotypic and phenotypic procedures and classified to thirteen genera, notably to the genera Micromonospora and Streptomyces. Actinomycetes showing cytotoxic activity were assigned to seven genera whereas only Micromonospora and Streptomyces strains showed anti-PTP1B activity. We conclude that actinomycetes isolated from mangrove habitats are a potentially rich source for the discovery of anti-infection and anti-tumor compounds, and of agents for treating neurodegenerative diseases and diabetes.
Keywords: Mangroves; actinomycete diversity; marine drug discovery; high-throughput screening; growth inhibition; protein tyrosine phosphatase 1B; aurora kinase A; caspase
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Hong, K.; Gao, A.-H.; Xie, Q.-Y.; Gao, H.G.; Zhuang, L.; Lin, H.-P.; Yu, H.-P.; Li, J.; Yao, X.-S.; Goodfellow, M.; Ruan, J.-S. Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China. Mar. Drugs 2009, 7, 24-44.
Hong K, Gao A-H, Xie Q-Y, Gao HG, Zhuang L, Lin H-P, Yu H-P, Li J, Yao X-S, Goodfellow M, Ruan J-S. Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China. Marine Drugs. 2009; 7(1):24-44.
Hong, Kui; Gao, An-Hui; Xie, Qing-Yi; Gao, Hao Gao; Zhuang, Ling; Lin, Hai-Peng; Yu, Hai-Ping; Li, Jia; Yao, Xin-Sheng; Goodfellow, Michael; Ruan, Ji-Sheng. 2009. "Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China." Mar. Drugs 7, no. 1: 24-44.