Next Article in Journal
Exploring the Valuable Carotenoids for the Large-Scale Production by Marine Microorganisms
Next Article in Special Issue
Sorbicillinoid-Based Metabolites from a Sponge-Derived Fungus Trichoderma saturnisporum
Previous Article in Journal
Structural Characterisation of Predicted Helical Regions in the Chironex fleckeri CfTX-1 Toxin
Previous Article in Special Issue
HSQC-TOCSY Fingerprinting-Directed Discovery of Antiplasmodial Polyketides from the Marine Ascidian-Derived Streptomyces sp. (USC-16018)
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Mar. Drugs 2018, 16(6), 202; https://doi.org/10.3390/md16060202

Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells

1
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
2
Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China
3
College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 4 May 2018 / Revised: 23 May 2018 / Accepted: 6 June 2018 / Published: 7 June 2018
(This article belongs to the Special Issue Marine Natural Products from Symbiotic Ecosystems)
View Full-Text   |   Download PDF [2989 KB, uploaded 7 June 2018]   |  

Abstract

Butyrolactone-I (ZB5-1) from the coral-derived fungus Aspergillus terreus was investigated in this study to estimate its anti-neuroinflammatory effects on lipopolysaccharide (LPS)-induced BV-2 microglia cells. MTT assay indicated that ZB5-1 in tested concentrations had no cytotoxicity on BV-2 cells, and significantly reduced the production of nitric oxide (NO), measured using Griess reagent, and interleukin-1 beta (IL-1β), detected by enzyme-linked immunosorbent assay (ELISA). ZB5-1 also down-regulated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner by Western blot analysis. Moreover, the effect of ZB5-1 on the nuclear factor-κB (NF-κB) signaling pathway was studied via the expression of phosphorylation of NF-κB p65 and inhibitor of NF-κB (IκB), and the nuclear translocation of NF-κB p65 respectively. The results showed that ZB5-1 could inhibit the phosphorylation of p65 and IκB. Furthermore, molecular docking study suggested that ZB5-1 bound at the active sites of NF-κB to prevent its translocation to the nucleus. Therefore, we suggest ZB5-1 has a potential to reduce the anti-inflammatory response in LPS-induced BV-2 cells. View Full-Text
Keywords: butyrolactone-I; Aspergillus terreus; microglia; neuro-inflammation butyrolactone-I; Aspergillus terreus; microglia; neuro-inflammation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Zhang, Y.Y.; Zhang, Y.; Yao, Y.-B.; Lei, X.-L.; Qian, Z.-J. Butyrolactone-I from Coral-Derived Fungus Aspergillus terreus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells. Mar. Drugs 2018, 16, 202.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top