Determination of the Deacetylation Degree of Chitooligosaccharides
AbstractThe methods for determination of chitosan content recommended in the Chinese Pharmacopoeia and the European Pharmacopoeia are not applicable for evaluation of the extent of deacetylation (deacetylation degree, DD) in chitooligosaccharides (COS). This study explores two different methods for assessment of DD in COS having relatively high and low molecular weights: an acid-base titration with bromocresol green indicator and a first order derivative UV spectrophotometric method for assessment of DD in COS. The accuracy of both methods as a function of molecular weight was also investigated and compared to results obtained using 1H NMR spectroscopy. Our study demonstrates two simple, fast, widely adaptable, highly precise, accurate, and inexpensive methods for the effective determination of DD in COS, which have the potential for widespread commercial applications in developing country. View Full-Text
Share & Cite This Article
Jiang, Y.; Fu, C.; Wu, S.; Liu, G.; Guo, J.; Su, Z. Determination of the Deacetylation Degree of Chitooligosaccharides. Mar. Drugs 2017, 15, 332.
Jiang Y, Fu C, Wu S, Liu G, Guo J, Su Z. Determination of the Deacetylation Degree of Chitooligosaccharides. Marine Drugs. 2017; 15(11):332.Chicago/Turabian Style
Jiang, Yao; Fu, Chuhan; Wu, Sihui; Liu, Guihua; Guo, Jiao; Su, Zhengquan. 2017. "Determination of the Deacetylation Degree of Chitooligosaccharides." Mar. Drugs 15, no. 11: 332.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.