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Mar. Drugs 2017, 15(1), 3; doi:10.3390/md15010003

Novel Peptide with Specific Calcium-Binding Capacity from Schizochytrium sp. Protein Hydrolysates and Calcium Bioavailability in Caco-2 Cells

1
The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry, Fuzhou University, Fuzhou 350108, China
2
College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China
*
Author to whom correspondence should be addressed.
Academic Editor: Se-Kwon Kim
Received: 1 October 2016 / Revised: 19 December 2016 / Accepted: 20 December 2016 / Published: 27 December 2016
(This article belongs to the Special Issue Marine Proteins and Peptides)
View Full-Text   |   Download PDF [2522 KB, uploaded 29 December 2016]   |  

Abstract

Peptide-calcium can probably be a suitable supplement to improve calcium absorption in the human body. In this study, a specific peptide Phe-Tyr (FY) with calcium-binding capacity was purified from Schizochytrium sp. protein hydrolysates through gel filtration chromatography and reversed phase HPLC. The calcium-binding capacity of FY reached 128.77 ± 2.57 μg/mg. Results of ultraviolet spectroscopy, fluorescence spectroscopy, and infrared spectroscopy showed that carboxyl groups, amino groups, and amido groups were the major chelating sites. FY-Ca exhibited excellent thermal stability and solubility, which were beneficial to be absorbed and transported in the basic intestinal tract of the human body. Moreover, the calcium bioavailability in Caco-2 cells showed that FY-Ca could enhance calcium uptake efficiency by more than three times when compared with CaCl2, and protect calcium ions against dietary inhibitors, such as tannic acid, oxalate, phytate, and Zn2+. Our findings further the progress of algae-based peptide-calcium, suggesting that FY-Ca has the potential to be developed as functionally nutraceutical additives. View Full-Text
Keywords: Schizochytrium sp.; protein hydrolysate; calcium-binding peptide; structure; bioavailability Schizochytrium sp.; protein hydrolysate; calcium-binding peptide; structure; bioavailability
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Cai, X.; Lin, J.; Wang, S. Novel Peptide with Specific Calcium-Binding Capacity from Schizochytrium sp. Protein Hydrolysates and Calcium Bioavailability in Caco-2 Cells. Mar. Drugs 2017, 15, 3.

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