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Mar. Drugs 2016, 14(3), 53; doi:10.3390/md14030053

Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum

Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
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Academic Editors: Andrew Desbois and Valerie Smith
Received: 21 December 2015 / Revised: 24 February 2016 / Accepted: 26 February 2016 / Published: 9 March 2016
(This article belongs to the Special Issue Marine Fatty Acids-2016)
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Abstract

We have created via metabolic engineering a heterotrophic strain of Phaeodactylum tricornutum that accumulates enhanced levels of the high value omega-3 long chain polyunsaturated fatty acid (LC-PUFAs) docosahexaenoic acid (DHA). This was achieved by generation of transgenic strains in which the Δ5-elongase from Ostreococcus tauri was co-expressed with a glucose transporter from the moss Physcomitrella patens. This double transformant has the capacity to grow in the dark in liquid medium supplemented with glucose and accumulate substantial levels of omega-3 LC-PUFAs. The effects of glucose concentrations on growth and LC-PUFA production of wild type and transformed strains cultivated in the light and dark were studied. The highest omega-3 LC-PUFAs accumulation was observed in cultures grown under mixotrophic conditions in the presence of 1% glucose (up to 32.2% of total fatty acids, TFA). Both DHA and EPA are detected at high levels in the neutral lipids of transgenic cells grown under phototrophic conditions, averaging 36.5% and 23.6% of TFA, respectively. This study demonstrates the potential for P. tricornutum to be developed as a viable commercial strain for both EPA and DHA production under mixo- and heterotrophic conditions. View Full-Text
Keywords: microalgae; Phaeodactylum tricornutum; omega-3 long chain polyunsaturated fatty acid; EPA; DHA; heterotrophic microalgae; Phaeodactylum tricornutum; omega-3 long chain polyunsaturated fatty acid; EPA; DHA; heterotrophic
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hamilton, M.L.; Powers, S.; Napier, J.A.; Sayanova, O. Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum. Mar. Drugs 2016, 14, 53.

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