Next Article in Journal
Astaxanthin as a Potential Neuroprotective Agent for Neurological Diseases
Next Article in Special Issue
Gliotoxin Inhibits Proliferation and Induces Apoptosis in Colorectal Cancer Cells
Previous Article in Journal
Dolch, L.-J. and Maréchal, E. Inventory of Fatty Acid Desaturases in the Pennate Diatom Phaeodactylum tricornutum. Mar. Drugs 2015, 13, 1317–1339
Previous Article in Special Issue
Sphingosines Derived from Marine Sponge as Potential Multi-Target Drug Related to Disorders in Cancer Development
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2015, 13(9), 5736-5749; doi:10.3390/md13095736

Development and Application of a Novel SPE-Method for Bioassay-Guided Fractionation of Marine Extracts

1
Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry-CNR, Via Campi Flegrei 34, Pozzuoli, Naples 80078, Italy
2
Marine Biotechnology Laboratory, Integrative Marine Ecology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy
3
GSK Vaccines s.r.l., via Fiorentina 1, Siena 53100, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Orazio Taglialatela-Scafati
Received: 16 June 2015 / Revised: 15 August 2015 / Accepted: 28 August 2015 / Published: 11 September 2015
(This article belongs to the Special Issue Marine Secondary Metabolites)
View Full-Text   |   Download PDF [737 KB, uploaded 11 September 2015]   |  

Abstract

The biological diversity of marine habitats is a unique source of chemical compounds with potential use as pharmaceuticals, cosmetics and dietary supplements. However, biological screening and chemical analysis of marine extracts pose specific technical constraints and require adequate sample preparation. Here we report an improved method on Solid Phase Extraction (SPE) to fractionate organic extracts containing high concentration of salt that hampers the recovery of secondary metabolites. The procedure uses a water suspension to load the extracts on a poly(styrene-divynylbenzene)-based support and a stepwise organic solvent elution to effectively desalt and fractionate the organic components. The novel protocol has been tested on MeOH-soluble material from three model organisms (Reniera sarai, Dendrilla membranosa and Amphidinium carterae) and was validated on a small panel of 47 marine samples, including sponges and protists, within discovery programs for identification of immuno-stimulatory and anti-infective natural products. View Full-Text
Keywords: drug discovery; bioactive marine natural products; SPE fractionation methods drug discovery; bioactive marine natural products; SPE fractionation methods
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Cutignano, A.; Nuzzo, G.; Ianora, A.; Luongo, E.; Romano, G.; Gallo, C.; Sansone, C.; Aprea, S.; Mancini, F.; D'Oro, U.; Fontana, A. Development and Application of a Novel SPE-Method for Bioassay-Guided Fractionation of Marine Extracts. Mar. Drugs 2015, 13, 5736-5749.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top