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Mar. Drugs 2015, 13(6), 3325-3339; doi:10.3390/md13063325

Influence of Core Oligosaccharide of Lipopolysaccharide to Outer Membrane Behavior of Escherichia coli

1,2
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2
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2
,
1,2
and
1,2,3,*
1
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
2
Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
3
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Academic Editor: Antonio Molinaro
Received: 14 April 2015 / Revised: 10 May 2015 / Accepted: 19 May 2015 / Published: 27 May 2015
(This article belongs to the Special Issue Marine Lipopolysaccharides)
View Full-Text   |   Download PDF [1167 KB, uploaded 27 May 2015]   |  

Abstract

Lipopolysaccharides, major molecules in the outer membrane of Gram-negative bacteria, play important roles on membrane integrity of the cell. However, how the core oligosaccharide of lipopolysaccharide affect the membrane behavior is not well understood. In this study, the relationship between the core oligosaccharide of lipopolysaccharide and the membrane behavior was investigated using a series of Escherichia coli mutants defective in genes to affect the biosynthesis of core oligosaccharide of lipopolysaccharide. Cell surface hydrophobicity, outer membrane permeability, biofilm formation and auto-aggregation of these mutant cells were compared. Compared to the wild type W3110, cell surface hydrophobicities of mutant ΔwaaC, ΔwaaF, ΔwaaG, ΔwaaO, ΔwaaP, ΔwaaY and ΔwaaB were enhanced, outer membrane permeabilities of ΔwaaC, ΔwaaF, ΔwaaG and ΔwaaP were significantly increased, abilities of biofilm formation by ΔwaaC, ΔwaaF, ΔwaaG, ΔwaaO, ΔwaaR, ΔwaaP, ΔwaaQ and ΔwaaY decreased, and auto-aggregation abilities of ΔwaaC, ΔwaaF, ΔwaaG, ΔwaaO, ΔwaaR, ΔwaaU, ΔwaaP and ΔwaaY were strongly enhanced. These results give new insight into the influence of core oligosaccharide of lipopolysaccharide on bacterial cell membrane behavior. View Full-Text
Keywords: lipopolysaccharide; core oligosaccharide; cell surface hydrophobicity; outer membrane permeability; biofilm formation; auto-aggregation; Escherichia coli lipopolysaccharide; core oligosaccharide; cell surface hydrophobicity; outer membrane permeability; biofilm formation; auto-aggregation; Escherichia coli
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, Z.; Wang, J.; Ren, G.; Li, Y.; Wang, X. Influence of Core Oligosaccharide of Lipopolysaccharide to Outer Membrane Behavior of Escherichia coli. Mar. Drugs 2015, 13, 3325-3339.

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