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Mar. Drugs 2015, 13(4), 2233-2249; doi:10.3390/md13042233

Molecular and Chemical Analysis of the Lipopolysaccharide from Aeromonas hydrophila Strain AH-1 (Serotype O11)

1
Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, 08071 Barcelona, Spain
2
Institute of Integrative Biology, University of Liverpool, Crown Street, L69 7ZB Liverpool, UK
3
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow V-334, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Antonio Molinaro
Received: 27 February 2015 / Revised: 30 March 2015 / Accepted: 7 April 2015 / Published: 14 April 2015
(This article belongs to the Special Issue Marine Lipopolysaccharides)
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Abstract

A group of virulent Aeromonas hydrophila, A. sobria, and A. veronii biovar sobria strains isolated from humans and fish have been described; these strains classified to serotype O11 are serologically related by their lipopolysaccharide (LPS) O-antigen (O-polysaccharide), and the presence of an S-layer consisting of multiple copies of a crystalline surface array protein with a molecular weight of 52 kDa in the form of a crystalline surface array which lies peripheral to the cell wall. A. hydrophila strain AH-1 is one of them. We isolated the LPS from this strain and determined the structure of the O-polysaccharide, which was similar to that previously described for another strain of serotype O11. The genetics of the O11-antigen showed the genes (wbO11 cluster) in two sections separated by genes involved in biosynthesis and assembly of the S-layer. The O11-antigen LPS is an example of an ABC-2-transporter-dependent pathway for O-antigen heteropolysaccharide (disaccharide) assembly. The genes involved in the biosynthesis of the LPS core (waaO11 cluster) were also identified in three different chromosome regions being nearly identical to the ones described for A. hydrophila AH-3 (serotype O34). The genetic data and preliminary chemical analysis indicated that the LPS core for strain AH-1 is identical to the one for strain AH-3. View Full-Text
Keywords: Aeromonas; genomics; proteomics; lipopolysaccharide; O11-antigen; LPS-core Aeromonas; genomics; proteomics; lipopolysaccharide; O11-antigen; LPS-core
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Merino, S.; Canals, R.; Knirel, Y.A.; Tomás, J.M. Molecular and Chemical Analysis of the Lipopolysaccharide from Aeromonas hydrophila Strain AH-1 (Serotype O11). Mar. Drugs 2015, 13, 2233-2249.

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