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Mar. Drugs 2014, 12(9), 4973-4983; doi:10.3390/md12094973

Additional Evidence of the Trypanocidal Action of (−)-Elatol on Amastigote Forms through the Involvement of Reactive Oxygen Species

1
Postgraduate Program in Pharmaceutical Sciences, State University of Maringa, Colombo Avenue 5790, Maringa, Parana CEP 87020-900, Brazil
2
Department of Marine Biology, Federal Fluminense University, P.O. Box 100644, Niteroi, Rio de Janeiro CEP 24001-970, Brazil
3
Department of Basic Health Sciences, State University of Maringa, Colombo Avenue 5790, Maringa, Parana CEP 87020-900, Brazil
*
Author to whom correspondence should be addressed.
Received: 24 January 2014 / Revised: 25 March 2014 / Accepted: 23 April 2014 / Published: 25 September 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
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Abstract

Chagas’ disease, a vector-transmitted infectious disease, is caused by the protozoa parasite Trypanosoma cruzi. Drugs that are currently available for the treatment of this disease are unsatisfactory, making the search for new chemotherapeutic agents a priority. We recently described the trypanocidal action of (−)-elatol, extracted from the macroalga Laurencia dendroidea. However, nothing has been described about the mechanism of action of this compound on amastigotes that are involved in the chronic phase of Chagas’ disease. The goal of the present study was to evaluate the effect of (−)-elatol on the formation of superoxide anions (O2), DNA fragmentation, and autophagy in amastigotes of T. cruzi to elucidate the possible mechanism of the trypanocidal action of (−)-elatol. Treatment of the amastigotes with (−)-elatol increased the formation of O2•− at all concentrations of (−)-elatol assayed compared with untreated parasites. Increased fluorescence was observed in parasites treated with (−)-elatol, indicating DNA fragmentation and the formation of autophagic compartments. The results suggest that the trypanocidal action of (−)-elatol might involve the induction of the autophagic and apoptotic death pathways triggered by an imbalance of the parasite’s redox metabolism. View Full-Text
Keywords: (−)-elatol; Laurencia dendroidea; Trypanosoma cruzi; Chagas’ disease; reactive oxygen species (−)-elatol; Laurencia dendroidea; Trypanosoma cruzi; Chagas’ disease; reactive oxygen species
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Desoti, V.C.; Lazarin-Bidóia, D.; Sudatti, D.B.; Pereira, R.C.; Ueda-Nakamura, T.; Nakamura, C.V.; de Oliveira Silva, S. Additional Evidence of the Trypanocidal Action of (−)-Elatol on Amastigote Forms through the Involvement of Reactive Oxygen Species. Mar. Drugs 2014, 12, 4973-4983.

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