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Mar. Drugs 2014, 12(6), 3587-3607; doi:10.3390/md12063587
Article

Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima

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Received: 5 March 2014; in revised form: 4 April 2014 / Accepted: 17 April 2014 / Published: 11 June 2014
(This article belongs to the Special Issue Metabolites in Diatoms)
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Abstract: Bacteria are known to influence domoic acid (DA) production by Pseudo-nitzschia spp., but the link between DA production and physiology of diatoms requires more investigation. We compared a toxic P. multiseries to a non-toxic P. delicatissima, investigating links between DA production, physiological parameters, and co-occurring bacteria. Bacterial communities in cultures of both species were reduced by antibiotic treatment, and each of the diatoms was inoculated with the bacterial community of the other species. The physiology of P. delicatissima was minimally affected by the absence of bacteria or the presence of alien bacteria, and no DA was detected. P. multiseries grew faster without bacteria, did not produce a significant amount of DA, and exhibited physiological characteristics of healthy cells. When grown with alien bacteria, P. multiseries did not grow and produced more DA; the physiology of these cells was affected, with decreases in chlorophyll content and photosynthetic efficiency, an increase in esterase activity, and almost 50% mortality of the cells. The alien bacterial community had morphological and cellular characteristics very different from the original bacteria, and the number of free-living bacteria per algal cell was much higher, suggesting the involvement of bacteria in DA production.
Keywords: bacteria; domoic acid; physiology; Pseudo-nitzschia bacteria; domoic acid; physiology; Pseudo-nitzschia
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lelong, A.; Hégaret, H.; Soudant, P. Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima. Mar. Drugs 2014, 12, 3587-3607.

AMA Style

Lelong A, Hégaret H, Soudant P. Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima. Marine Drugs. 2014; 12(6):3587-3607.

Chicago/Turabian Style

Lelong, Aurélie; Hégaret, Hélène; Soudant, Philippe. 2014. "Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima." Mar. Drugs 12, no. 6: 3587-3607.



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