Mar. Drugs 2014, 12(5), 2397-2407; doi:10.3390/md12052397
Article

Development of Pedigree Classification Using Microsatellite and Mitochondrial Markers for Giant Grouper Broodstock (Epinephelus lanceolatus) Management in Taiwan

1,2,3,†email, 1,2,3,†email, 1,3email, 1,2email, 1,2,3email and 1,2,3,4,5,* email
Received: 10 March 2014; in revised form: 3 April 2014 / Accepted: 8 April 2014 / Published: 30 April 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Most giant groupers in the market are derived from inbred stock. Inbreeding can cause trait depression, compromising the animals’ fitness and disease resistance, obligating farmers to apply increased amounts of drugs. In order to solve this problem, a pedigree classification method is needed. Here, microsatellite and mitochondrial DNA were used as genetic markers to analyze the genetic relationships among giant grouper broodstocks. The 776-bp fragment of high polymorphic mitochondrial D-loop sequence was selected for measuring sibling relatedness. In a sample of 118 giant groupers, 42 haplotypes were categorized, with nucleotide diversity (π) of 0.00773 and haplotype diversity (HD) of 0.983. Furthermore, microsatellites were used for investigation of parentage. Six out of 33 microsatellite loci were selected as markers based on having a high number of alleles and compliance with Hardy-Weinberg equilibrium. Microsatellite profiles based on these loci provide high variability with low combined non-exclusion probability, permitting practical use in aquaculture. The method described here could be used to improve grouper broodstock management and lower the chances of inbreeding. This approach is expected to lead to production of higher quality groupers with higher disease resistance, thereby reducing the need for drug application.
Keywords: giant grouper; inbreeding; mitochondria; microsatellite
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MDPI and ACS Style

Kuo, H.-C.; Hsu, H.-H.; Chua, C.S.; Wang, T.-Y.; Chen, Y.-M.; Chen, T.-Y. Development of Pedigree Classification Using Microsatellite and Mitochondrial Markers for Giant Grouper Broodstock (Epinephelus lanceolatus) Management in Taiwan. Mar. Drugs 2014, 12, 2397-2407.

AMA Style

Kuo H-C, Hsu H-H, Chua CS, Wang T-Y, Chen Y-M, Chen T-Y. Development of Pedigree Classification Using Microsatellite and Mitochondrial Markers for Giant Grouper Broodstock (Epinephelus lanceolatus) Management in Taiwan. Marine Drugs. 2014; 12(5):2397-2407.

Chicago/Turabian Style

Kuo, Hsiao-Che; Hsu, Hao-Hsuan; Chua, Chee S.; Wang, Ting-Yu; Chen, Young-Mao; Chen, Tzong-Yueh. 2014. "Development of Pedigree Classification Using Microsatellite and Mitochondrial Markers for Giant Grouper Broodstock (Epinephelus lanceolatus) Management in Taiwan." Mar. Drugs 12, no. 5: 2397-2407.


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