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Mar. Drugs 2014, 12(1), 54-68; doi:10.3390/md12010054
Article

Palmitic Acid and Ergosta-7,22-dien-3-ol Contribute to the Apoptotic Effect and Cell Cycle Arrest of an Extract from Marthasterias glacialis L. in Neuroblastoma Cells

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Received: 18 October 2013; in revised form: 28 November 2013 / Accepted: 9 December 2013 / Published: 24 December 2013
(This article belongs to the Special Issue Marine Compounds and Cancer)
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Abstract: We describe the effect of a chemically characterized lipophilic extract obtained from Marthasterias glacialis L. against human breast cancer (MCF-7) and human neuroblastoma (SH-SY5Y) cell lines. Evaluation of DNA synthesis revealed that both cell lines were markedly affected in a concentration-dependent way, the SH-SY5Y cell line being more susceptible. Cell cycle arrest was observed, an effect induced by the sterol, ergosta-7,22-dien-3-ol, present in the extract. Morphological evaluation of treated cells showed the advent of lipid droplets and chromatin condensation compatible with apoptosis, which was confirmed by the evaluation of caspase-3 and -9 activities. Palmitic acid was the main compound responsible for this apoptotic effect by a ceramide-independent mechanism that involved endoplasmic reticulum (ER)-stress with upregulation of CCAAT/-enhancer-binding protein homologous protein (CHOP).
Keywords: Marthasterias glacialis L.; palmitic acid; ER-stress; CHOP; apoptosis Marthasterias glacialis L.; palmitic acid; ER-stress; CHOP; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pereira, D.M.; Correia-da-Silva, G.; Valentão, P.; Teixeira, N.; Andrade, P.B. Palmitic Acid and Ergosta-7,22-dien-3-ol Contribute to the Apoptotic Effect and Cell Cycle Arrest of an Extract from Marthasterias glacialis L. in Neuroblastoma Cells. Mar. Drugs 2014, 12, 54-68.

AMA Style

Pereira DM, Correia-da-Silva G, Valentão P, Teixeira N, Andrade PB. Palmitic Acid and Ergosta-7,22-dien-3-ol Contribute to the Apoptotic Effect and Cell Cycle Arrest of an Extract from Marthasterias glacialis L. in Neuroblastoma Cells. Marine Drugs. 2014; 12(1):54-68.

Chicago/Turabian Style

Pereira, David M.; Correia-da-Silva, Georgina; Valentão, Patrícia; Teixeira, Natércia; Andrade, Paula B. 2014. "Palmitic Acid and Ergosta-7,22-dien-3-ol Contribute to the Apoptotic Effect and Cell Cycle Arrest of an Extract from Marthasterias glacialis L. in Neuroblastoma Cells." Mar. Drugs 12, no. 1: 54-68.



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