The world capture of marine organisms including aquaculture (mainly fish, mollusks and crustaceans) amounts to 132 million tons [1]. Among them, more than 35% of the total weight is handled as by-product and waste that include animal fractions (skeletons, heads, viscera) generated in seafood production or species, sizes or qualities without commercial value (discards and by-catch). Commonly, the production of such wastes is located in coastline areas with the corresponding problem associated with environmental pollution generated by an inefficient residue management [2,3]. In addition, the overexploitation of several species (e.g., sharks) has led to ecological risks derived from the reduction of biological resources [4]. To establish a more efficient control of fisheries, to increase the profitability of seafood operations and to satisfy environmental regulations, low cost and environmentally friendly technologies are being evolved by the necessity of recovering all the materials (polysaccharides, proteins, oils, minerals) [3,5,6,7]. Recently, alternative methods have been developed to obtain different products and molecules: enzymes, glycosaminoglycans, chitin, gelatin, biosilage, marine peptones, etc., from skeletons, skins, viscera, heads, etc. [8,9,10,11,12,13,14,15,16,17,18,19,20,21,22]. Bearing in mind the number of applications and the economical value of final products, glycosaminoglycans and chitin are two of the most important and relevant compounds to upgrade from marine wastes [23].

Glycosaminoglycans (GAGs) are heteropolysaccharides defined by a repeating disaccharide unit without branched chains in which one of the two monosaccharides is always an amino sugar (N-acetylgalactosamine or N-acetylglucosamine) and the other one is a uronic acid. They are present on all animal cell surfaces and in the extracellular matrix where are known to bind and regulate different proteins (e.g., growth factors, enzymes, cytokines). After purification, they are used in numerous contexts from food, cosmetic and clinical areas [24,25,26].

Chemical structure of chitin is also a long linear chain formed by successive units of an amino monosaccharide (N-acetylglucosamine); however, it is not commonly classified as GAGs. It is the second most extended polysaccharide in nature after cellulose, forming part of microorganism cell walls, exoskeleton of insects and shells of crustaceans. Both chitin and its partially deacetylated form chitosan have been intensely studied in recent years with a promising potential for applications in pharmacy, alimentary and biomedicine devices [27,28,29].

Several methodologies have been developed to produce the mentioned biopolymers prepared with steps of hydrolysis and purification that are usually expensive and/or environmentally not friendly, for instance, to manage large volumes of alkalis and strong acids needed in hydrolysis and to use specific chromatographic techniques hardly scale-up in purification. The present review addresses an overview of different sustainable and clean processes to recover chondroitin sulfate (CS), hyaluronic acid (HA) and chitin/chitosan (CH/CHs) from marine waste materials.

Traditionally, the production of GAGs is obtained from mammalian tissues mainly generated in slaughterhouse: Rooster combs, cartilage (tracheas and nasal from bovine and swine) and umbilical cords. However, as a consequence of the concern due to the bovine spongiform encephalopathy (BSE) and other food chain crisis, the exploration of microorganism and marine organisms as source of those glycoconjugates has received increasing attention. Marine organisms like sponges, sea cucumbers, squids, mollusks, invertebrates and mainly cartilaginous material from fishes (shark, salmon, ray, etc.) are well-documented as potential producers of them [30,31,32].

Cartilage is a tissue formed by a matrix of collagen associated with proteoglycans, macromolecules with a core protein to which the GAGs chondroitin sulfate, keratan sulfate, dermatan sulfate and heparan sulfate are covalently attached by means of a trisaccharide linked to a serine residue. HA is the only non-sulfated GAGs and is not covalently bound to the protein in any tissue, although specific HA-protein interaction is shown [33]. CS and HA are the most valued GAGs in market because of its abundance in mammalian tissues, physiological functions and high activity.

The types of applications for the formulations of CS or CS-derived, and therefore their market price, are dependent on the concentration and purity of this GAG in the commercial products. Different compounds including chemical solvents and detergents from isolation step and peptides, proteins, nucleic acids or organic compounds from tissues are commonly contaminating the samples; hence, they are reducing its commercial value and limiting its usage areas [49]. Clinical applications demand highly concentrated and pure CS in comparison with cosmetic, dietary supplements or food ingredients. Moreover, CS derived from fish (ray and shark) is referred as a better source than mammalian because of its sulfation pattern and safety. Therefore, it is especially important the development of highly yielded and low-cost extraction processes, maintaining the quality and great purity of CS in order to execute an optimum exploitation of marine sources.

In general, the methods of CS isolation from cartilage (the most interesting substrate from an industrial viewpoint) are defined for several years [59,60,61] and include various steps based on: (1) chemical hydrolysis of cartilage; (2) breakdown of proteoglycan core; (3) elimination of proteins and CS recovery; (4) purification of CS. The two first stages are mostly conducted by means of alkaline hydrolysis at high concentrations of NaOH, urea or guanidine HCl, subsequently combined with selective precipitation of GAG using cationic quaternary ammonium chemicals (as cetylpyridinium chloride), potassium thiocyanate, non-ionic detergents or alcoholic solutions [59,60], deproteinization by trichloroacetic acid and finally purification with gel filtration and/or ion-exchange and size-exclusion chromatography [62]. Unfortunately, those economically viable stages lead to unsatisfactory purity for clinical uses of CS. The techniques that improve final product quality need larger amounts of reagents and are time-consuming. In addition, costumers and manufactures try to develop more environmentally friendly and economical processes to obtain CS based on non contaminant solvent strategies.

Various alternative isolation methods have been recently developed to replace the classical methods for pursuing sustainability [63,64,65,66]. Those processes can be summarized as follows: digestion of cartilage and proteins mediated by enzymes, selective precipitations with alcoholic solutions, resuspension and neutralization with salt solutions and separation by molecular-weight using ultrafiltration-diafiltration technologies (UF-DF). Figure 1 shows a flow chart representing all the potential steps described for the downstream purification of marine CS. Firstly, the fishing by-products (e.g., ray skeletons or shark heads) are warmed separating the rests of flesh, excellent material for fish meal, and cartilage for CS production. Subsequently, dried and milled cartilage is hydrolyzed by proteases under controlled experimental conditions. Multiple enzymes have been studied, generally with successful results, with the objective of cartilage degradation, protein fraction breakdown and to obtain undamaged CS molecules. The proteolysis of proteoglycans from hammerhead shark fin cartilage was partially degraded by commercial papain but trypsin or superase were not effective [67]. Similar activity of papain digestion was also observed in adult zebrafish [68], ray [35] and dogfish tissues [69]. In all cases, the time of hydrolysis was superior to 18 h under optimal conditions of temperature (50–65 °C) and pH 7. Recently, a two-step enzymatic processing with alcalase and flavourzyme showed better yields of degradation with a significant reduction of time-processing [21]. Proteolytic and collagenolytic activities isolated from skate pancreas led to percentage of skate cartilage hydrolysis higher than 50% in 6 h [66]. The separation of hydrolysates is generally carried out by simple decantation or centrifugation removing the supernatant rich in CS and the rests of cartilage precipitated (useful as substrate for fish meal production). Tadashi [65] suggested a previous elimination stage of cartilage wastes based on the addition of activated charcoal at 55 °C.

The subsequent phase of alcoholic treatment is usually indicated by several authors as crucial for the selective precipitation of CS from the major protein presents in the hydrolysate [61,65,66,70]. The effectiveness of that process is dependent on the type and alcohol concentration and, in some cases, the influence of processing-temperature is also important [65]. Ethanol is the most commonly selected reagent for such precipitation, at concentrations of 40%–60%, due to its widespread use as a solvent of substances intended for human contact or consumption [65,66,70]. A recent report also proposed isopropanol at 40% for the purification of CS from scapular cartilage of shortfin mako shark [21]. In our lab, we have optimized the combination of alkaline proteolysis and selective precipitation of CS from ray cartilage by using alkaline hydroalcoholic solutions [66]. Under optimal conditions of NaOH 0.2 M and one volume of ethanol per volume of hydrolysate at room temperature with soft agitation for 1 h, more than 96% of CS recovery and CS purity were obtained. The repetition of this procedure, under the same experimental conditions, increased the CS purity up to 99%. The resuspension of CS sediment and pH neutralization is efficiently obtained by means of saline solutions as NaCl or sodium acetate, the salt excess can be subsequently removed by membrane dialysis method.

The last step of purification by membrane technologies is widely performed in the majority of the biomacromolecules downstream processing (with higher sizes of 1 kDa) because of its separation effectiveness, easy scale-up, cost effective device, numerous types and cut-off membranes and simple operatory and control. However, the use of described methodology, to remove low-molecular-weight materials and salts from neutralized or resupended CS solution, has been poorly studied. Large quantities of CS from salmon tissues were extracted by alkali treatment and subsequent purified by repeating UF procedure, demonstrating superior efficient than using ion exchange resin [71]. A two-step process based on enzyme extraction of CS and concentration-desalting by UF-DF was studied using skate cartilage as substrate and ceramic membranes [63]. The authors advised that the desalting step by DF should be improved with a higher filtering area due to the 40% remaining of salts in the final solution of CS. This inconvenience was improved via polyethersulfone membranes with molecular-weight cut-off at 10 kDa and 6 diavolume [66]. The UF-DF system was assembled with total recirculation to obtain CS of 99.6% purity at final concentration of 35–45 g/L. Higher CS concentrated liquid generated excessive viscosities that reduced the filtrate flow and filled the membranes.

Finally, the powder of CS can be obtained by drying the concentrated solutions without adding chemical solvents using spray dryer equipment or evaporation in an oven, depositing CS in thin layers on trays followed by milling performance.

The most conventional materials employed for HA extraction are selected for its feasibility and concentration, thus, umbilical cord presents an average level of 4 g/L, synovial fluid from pig (3 g/L) or bovine (18 g/L) and rooster combs (25 g/L) [56,81,82,83]. Nevertheless, the risk of animal-derived pathogens, inter-species viral or prionic contaminations (e.g., BSE, epizootic aphtha) has obligated to explore and optimize other alternatives of production.

In marine organisms, the only clear source of HA is the VH present in the eyeball of fish species. VH volume and HA concentration is different depending on the selected fish, for instance, HA is obtained from eyeballs of shark and swordfish at 0.3 g/L from 18 mL of VH and 0.055 g/L from 70 mL, respectively [56]. HA is also present in the cartilage matrix in which is very important as structural element of the aggrecan in cartilaginous fishes; however, its relatively low content makes it economically unavailable for any industrial extraction process [84].

The traditional protocols for extraction of HA from animal substrates (e.g., rooster comb) are developed according to the works reported by Swann [85] and Balazs [86] that include the preparation of the material, washing with water or alcohol, aqueous or organic solvent (mainly chloroform) extraction, precipitation by cetylpiridinium chloride, filtering and successive extractions with chloroform, centrifugation and occasional chromatographic purification. Other authors indicated that both procedures are costly, laborious, time-consuming and lead to contaminated HA solutions that limit their applications in biopharma formulations [87].

Different strategies have been proposed for the extraction of HA from mammalian VH including deproteinization of bovine substrate with xylenesulphonate-Na [88], extraction with water, precipitation with ethanol and purification by DEAE-cellulose [89] and fractional precipitation using ethanol combined with enzymatic protein hydrolysis [59]. The most exhaustive method was applied to tuna eyeball substrate [90], performing precipitation with overcooled acetone, actinase digestion, thermal coagulation, dialization by membrane and cetylpyridinium chloride precipitation. Similarly, we have addressed a method to recover and purify HA of VH from various fish eyeballs (tuna, shark, and swordfish) using easier, faster and cheaper stages [56]: (1) initial clarification of VH extracted from frozen eyeball (using centrifugation or glass wool filtration); (2) enzyme proteolysis; (3) concentration by UF; (4) precipitation and alkaline proteolysis in hydroalcoholic medium at low temperature; (5) selective redissolution and neutralization; (6) separation, purification and concentration by UF-DF; (7) removing of nucleic acids using absorption with hydroxyapatite obtained from fish bone. Medical-grade purity of 99.9% (molecular weight 2000 kDa) was thus reached (Figure 2). The lyophilization of final solutions is an excellent lab resource to avoid physical degradation and size reduction but they are too expensive for industrial scale.

Although prices of vitreous HA are commercially high, economic viability is unclear given the cost of vitreous humor (VH) removal (expensive labor) and the overdependence on raw materials (opportunistic prices of fish eyes) that usually tends to the overexploitation of this marine resource. Microbial production easily contains the concentration equivalent (3 g/L) to 660 eyes of shark or 900 of swordfish. Additionally, the presence of blood in the eyes supply should be avoided as much as possible because the iron from haemoglobin degrades HA molecule.

During recent years, CH and CHs have attracted a great interest due to their distinctive biological and physicochemical properties [28], which make them interesting polymers, among others, for biotechnology, medicine, cosmetics, food technology and textile applications.

Marine organisms are principal source of CH since it is a constituent of the organic matrix of the exoskeletons of arthropods such as crustaceans (crabs, lobsters and shrimps) and of the endoskeleton of mollusks [103]. Although CH can also be found in many other organisms including fungi [104], yeasts [105], algae and squid pen [106], the shell of marine crustaceans is the preferred source of CH due to their high availability as waste from the seafood processing industry [28].

However, traditional methods involved in the recovery of CH from shellfish are extremely hazardous, energy consuming and environmentally polluting, due to the need of using high amounts of mineral acid and alkali [107]. In addition, the deacetylation of CH to produce CHs requires the use of very intense alkaline treatments. Hence, alternative environmentally friendly processes are being assessed including the use of proteases or proteolytic bacteria for deproteinization and demineralization of crustacean shells [108,109]. Alternatively, fermentation using lactic acid bacteria (LAB) has been widely applied for the extraction of CH from crab [110,111] and shrimp [112,113] biowastes. In case of CHs production, fungus Mucor rouxii has been widely studied as an alternative source of chitosan.

The most common sources of CH are crab and shrimp shell wastes. In the skeletal tissue of these species, CH is bound to proteins forming a chitin-protein matrix associated to mineral salts [11], principally calcium carbonate. In this regard, the main components of crustacean shells are on a dry weight basis and depending on the species and season, 30%–40% protein, 30%–50% mineral salts and 13%–42% CH [28]. Furthermore, small amounts of lipids from muscle or viscera residues [117] and carotenoids, mainly astaxanthin and is esters [131], associated with proteins of the exoskeleton can be found in crustacean shell waste.

The traditional method for the industrial recovery of CH from different crustacean shells consists of two steps (Figure 3), including a deproteinization with alkali treatment at high temperatures and a demineralization using diluted hydrochloric acid as the preferred reagent. Although it is considered that the order of these two phases is interchangeable depending on the source and proposed use of chitin [117], other authors suggest that demineralization should be performed first in order to decrease the residual mineral content [132].

After demineralization and deproteinization, CH isolated from crustacean sources has a lightly pink color and so a bleaching process using potassium permanganate, oxalic acid [133] or hydrogen peroxide [132] is usually carried out to yield a colorless product. On the contrary, CH isolated from squid pens is completely white and therefore, this final stage is unnecessary.

It is generally accepted that the processing conditions significantly affect the molecular weight and acetylation degree of CH. In this sense, the stronger the acidic conditions utilized for demineralization (pH, time and temperature), the lower molecular weight products are obtained [11]. Percot et al. studied the kinetics of demineralization of shrimp shells by following the pH variations in the reaction medium [11]. According to their results, they were able to define the optimal conditions necessary to perform a complete reaction, minimizing the hydrolysis of the glycosidic bonds. For this purpose, an excess of 0.25 M HCl, a solid-to-liquid ratio above 10 mL/g and 15 min of reaction at ambient temperature provided a final product with a DA above 95%.

In contrast, deproteinization by alkaline treatment has shown to be less damaging to the chitin structure compared to the acidic treatment involved in the demineralization [119]. In fact, Percot et al. reported that deproteinization using 1 M NaOH with a temperature and a reaction time below 70 °C and 24 h had no influence on both the molecular weight and DA, respectively [11]. Nevertheless, a large variation exists for the reported conditions of deproteinization for CH preparation. Chang and Tsai [134] analyzed protein removal from shrimp shell waste using NaOH by response surface methodology, reporting optimal conditions with 2.5 N NaOH, 75 °C and a minimal solution to solid ratio of 5 mL/g. According to their results, these authors also reported that kinetics of demineralization and deproteinization were pseudo-first order and two-stage first-order reactions, respectively. Tolaimate et al., using a tailored isolation process according to the source of CH (shrimp, crab, lobster or squid), were able to obtain highly acetylated products (near 100%) preserving the crystalline structure of both α and β chitin [132]. These authors using low concentrated acid (0.55 M HCl) and base (0.3 M NaOH) solutions in a multi-stage process, highlighted the need to adapt the process conditions to the origin and specific characteristics of the CH source utilized.

On the other hand, the most commonly used methods for CHs production are the Broussignac [135] and Kurita [106] processes. The first procedure consists of a deacetylation of chitin in a nearly anhydrous reaction medium using a mixture of potassium hydroxide, ethanol and monoethylene glycol. On the other hand, the Kurita method proceeds in a stirred aqueous solution of sodium hydroxide, under a nitrogen stream at high temperatures (>80 °C).

Tolaimate et al. extensively compared both deacetylation methods and these studies indicated that the adjustment of different parameters related to the deacetylation process, the nature of the source, physical structure of the original CH and its isolation process allow to prepare CHs with controlled physico-chemical (molecular weight and DA) characteristics either from α or β-chitins [132,136]. Comparing the two processes for the production of CHs from a completely N-acetylated β-chitin prepared from squid pen (Loligo vulgaris), these authors concluded that the Kurita process enabled to obtain CHs with high molecular weights and a wide range of deacetylation degrees [136]. On the contrary, the Broussignac process could be carried out to obtain CHs with low degrees of acetylation and molecular weights, but in a faster way. Nevertheless, due to the high amounts of alkali and acid wastewaters generated in these production processes, there is a need to find alternatives to overcome the problem of wastewater neutralization. A possible way that has not been sufficiently explored to date is the reutilization of these effluents in the alkaline proteolysis step of CS isolation from cartilage (Figure 1). This strategy would allow the recycling of highly polluting wastewaters and goes towards the overall utilization of marine by-products.

Chemical CH purification is an energy consuming process and results in environmental problems with high waste processing costs, due to the need of neutralization of processing wastewaters [111]. Besides and as stated above, prolonged alkaline and acid treatments cause depolymerization and deacetylation of the polysaccharide. Furthermore, the low biological value of alkali-recovered proteins may limit its application in the animal feed industry, thus affecting the production costs of CH and CHs from crustacean by-products [117]. In recent years, several methods have been reported in the literature to solve chemical extraction problems (Figure 4). One of the biological alternatives proposed is the use of proteases for deproteinization of crustacean shells, avoiding alkaline treatments. Various commercial proteases have been assayed for protein removal from crustacean shells [137], being alcalase the most employed and effective enzyme [137,138,139]. In addition, the utilization of crude proteolytic extracts obtained from different microorganisms [140,141] or even from fish viscera [142] have been studied, leading to varying deproteinization yields depending on the conditions assayed. Although deproteinization levels achieved in such cases are generally lower than those obtained using alkaline treatments, this alternative has the advantage to produce nutritionally valuable protein hydrolysates in addition to chitin [138].

When using enzymatic deproteinization, previous demineralization is more convenient since it increases the permeability of the tissues and reduces the presence of potential enzyme inhibitors, favoring the subsequent action of the enzyme [138].

Another biotechnological approach for the production of CH from seafood wastes consists on their fermentation using lactic acid bacteria (LAB). The production of bio-silages from fish by-products consists on the ability of LAB strains to ferment the waste materials and to produce in situ organic acids, mainly lactic and acetic acids, in order to preserve and produce ingredients for animal feed production [18,102]. This methodology has also been applied for the recovery of other value-added by-products from ensilaged shrimp waste, such as carotenoids [143].

In the fermentation of crustacean by-products, two fractions are obtained: a solid phase containing crude chitin and a liquor fraction rich in proteins, minerals and pigments. This occurs because lactic acid produced during fermentation operates at two levels. On the one hand, it reacts with the calcium carbonate to produce calcium lactate, which precipitates and can be easily removed by washing [144]. In addition, lactic acid decreases pH values, leading to the activation of proteases. Deproteinization of the biowaste and simultaneous liquefaction of the proteins occurs mainly by proteolytic enzymes produced by the added LAB, by gut bacteria of the intestinal system of crustaceans, or by proteases present in the source byproduct [145].

Several LAB have been assayed in a wide range of raw materials of marine origin. Shrimp waste has been mainly fermented using Lactobacillus plantarum [113,146], but also with other lactic acid bacteria such as Lactobacillus paracasei [147], Pediococcus acidolactici [148] and Lactobacillus helveticus [149]. Non-LAB, including Pseudomonas aeruginosa K-187 [150] and Bacillus subtilis [151] have been assayed as inoculum source for the recovery of CH. Commercial bacterial inoculums containing a mixture of LAB have been utilized for the production of CH from waste shell of prawn (Nephrops norvegicus). Stabisil containing Streptococcus faecium M74, L. plantarum, and P. acidilactici [152] and a powdered grass silage inoculant consisting of a mixture of selected proteolytic enzyme producing bacteria [107] proved to be effective alternatives for the demineralization and deproteinization of prawn biowastes.

Duan et al. reported the production of CH from shrimp waste by fermentation with the epiphytic strain Lactobacillus acidophilus SW01 isolated from shrimp by-products [133]. Due to its high protease activity, the solid residue from fermented shrimp waste contained less than 1% minerals and proteins. Therefore, after 168 h of cultivation at 37 °C, pure CH could be easily recovered only following a bleaching treatment.

Co-fermentation using a LAB and a bacterium with proteolytic activity has also been investigated as an alternative for CH purification from marine by-products. The LAB Lactococcus lactis and Teredinobacter turnirae, a protease producer marine bacterium, were jointly utilized for the for biological CH extraction from prawn waste [153]. Both bacteria were cultivated individually and co-fermented in a culture medium prepared with 10% (w/v) shell solids in the presence of increasing concentrations of glucose (0%–15% w/v). Although the extraction of CH following this procedure was incomplete compared to the chemical method, the highest process yield (95.5%) was obtained when T. turnirae was first inoculated in co-fermentation. Similar results were obtained by Jung et al., who co-cultivated the lactic acid bacterium L. paracasei subsp. tolerans KCTC-3074 and the protease producing bacterium Serratia marcescens FS-3 in crab shells [154]. These authors founded that the co-fermentation process was efficient, although highlighted the need to improve deproteinization.

In a later paper, Jung et al. reported for the first time successive two-step fermentation from red crab shell wastes using the same species than in the previous work [111,154]. This research concluded that the sequential order of inoculation is an important issue, since the best results in co-removal of CaCO₃ and proteins, 94.3% and 68.9%, respectively, from crab shells were obtained when successive fermentation was carried out in a first step with S. marcescens followed by a second cultivation with L. paracasei, and not vice versa.

Several process variables have been reported to influence the fermentation of marine wastes and therefore the efficiency of CH recovery from these sources, such as inoculum ratio [147], temperature [155] and initial pH [146]. Also carbon source and level, and the carbon on nitrogen ratio [147,156] were found to be important parameters for CH recovery from crustacean shells. Although the majority of the reports use the one-factor-at-a-time approach to study the effect of these variables on fermentation performances, other studies have attempted to optimize fermentation conditions for chitin recovery using response surface methodology [148,149,155].

Nevertheless, from the stated above it follows that demineralization and deproteinization occur simultaneously but incompletely in these biological processes [111]. This lower performance of LAB fermentation in deproteinization and demineralization of shell waste has been attributed to the compact structure of the shells [113]. For this reason, the fermentation of crustacean shells has been reported as a complementary strategy to chemical treatments, leading to a decrease in the amount of corrosive chemicals in the CH extraction process [112]. In addition to the reduction in the use of reagents, a major advantage of the fermentation process is obtaining a high-value by-product in the form of liquor rich in protein, minerals and asthaxanthin [113].

The same manner as chemical CH purification, the production of CHs by deacetylation of crustacean chitin with strong alkali appears to have limited potential for industrial acceptance, because of the large amounts of concentrated alkaline solution waste causing environmental pollution. Moreover, the conversion of CH to CHs, using a strong base solution at high temperature, causes variability of the product properties, decreases the CHs quality and increases the processing costs [157]. An alternative source of CHs is the cell wall of fungi, mainly zygomycetes. Among them, the fungus M. rouxii has been reported to contain significant amounts of CHs, CH and acidic polysaccharides as cell wall components [158]. For this reason, bioproduction of CHs from M. rouxii has been widely studied during recent years [117,159,160,161,162]. According to Chatterjee et al. culture media and fermentation conditions can be varied to provide CHs of more consistent physico-chemical properties compared to that obtained by chemical modification of chitin [159]. Among three fungal culture media, molasses salt medium (MSM), potato dextrose broth (PDB) and yeast extract peptone glucose (YPG), chitosan from MSM was less polydispersed and more crystalline compared to those from YPG and PDB, thus indicating a higher quality of the polymer.

Since CHs is a constituent of M. rouxii cell walls, its production is coupled to fungal growth, and therefore maximal productions are obtained when mycelial growth is maximal. CHs molecular weight was found to be dependent on the growth phase of M. rouxii, showing an increase of molecular weight with time of culture [160]. These authors also found a great influence of the pH on fungal growth and therefore on CHs production. Trutnau et al. found a higher CHs content with increasing time of cultivation in semi-continuous cultures, suggesting an adaption of the fungi to shear stress [162]. According to these authors, their results and model predictions of hyphal growth, suggest that repeated batch cultures might be optimal for CHs production.

Naturally occurring CHs is produced in situ by enzymatic deacetylation of chitin [163]. CH deacetylases were characterized in various fungi, such as M. rouxii [164], Rhizopus nigricans [165] and Aspergillus nidulans [166]. These enzymes have been also explored as an alternative to alkali treatment on chitin production from crustacean shells. Nevertheless, fungal CH deacetylases studied so far are only able to perform enzymatic deacetylation on their solid substrate to a 5%–10% of the total N-acetylglucosamine residues [167], preferring N-acetylglucosamine homopolymers as substrates [164]. Therefore pretreatment of crystalline CH would be necessary prior to enzyme hydrolysis, in order to improve the accessibility of acetyl groups to the enzyme. Several physical and chemical methods such as heating, sonicating, grinding, derivatization and interaction with saccharides have been assayed in order to improve the accessibility to the acetyl groups for the deacetylation [168]. Win and Stevens were successful at deacetylating CH to CHs (10% DA), using a chitin deacetylase from the fungus Absidia coerulea [167]. In this work a pretreatment of superfine CH, a decrystallized form with a very small particle size, with 18% formic acid resulted in the nearly complete enzymatic deacetylation.

Finally it is important to note that besides allowing the reduction in the use of chemicals, fungal CHs possesses two advantages that are interesting for medical applications: a lower molecular weight and lower contents of heavy metals [162]. In Table 2, different microbial processes studied for CH and CHs from marine sources are reported.

CS, HA and CH/CHs have attracted increasing attention because of their beneficial effects on several ambits of the human health, in the formulation of cosmeceuticals and anti-aging products, nutraceuticals and food ingredients as well as their application in bio and nanotechnological processes. From long time ago, extensive studies have been conducted on the clarification of the general aspects of the chemical structures, features, novel applications and more sustainable processes for their production. In this review, we have discussed a set of recent progresses in the definition of eco-friendly processes to extract and purify those biomacromolecules from marine by-products.