Mar. Drugs 2013, 11(11), 4464-4477; doi:10.3390/md11114464
Article

Isolation and Structural Characterization of a Novel Antioxidant Mannoglucan from a Marine Bubble Snail, Bullacta exarata (Philippi)

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Received: 11 September 2013; in revised form: 12 October 2013 / Accepted: 12 October 2013 / Published: 11 November 2013
(This article belongs to the Special Issue Marine Polysaccharides)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Bullacta exarata is one of the most economically important aquatic species in China, noted for not only its delicious taste and nutritional value, but also for its pharmacological activities. In order to explore its potential in medical applications, a mannoglucan designated as BEPS-IB was isolated and purified from the foot muscle of B. exarata after papain digestion. Chemical composition analysis indicated BEPS-IB contained mainly d-glucose and d-mannose in a molar ratio of 1:0.52, with an average molecular weight of about 94 kDa. The linkage information was determined by methylation analysis, and the anomeric configuration and chain linkage were confirmed by IR and 2D NMR. The results indicated BEPS-IB was composed of Glcp6Manp heptasaccharide repeating unit in the backbone, with occasional branch chains of mannose residues (14%) occurring in the backbone mannose. Further antioxidant assay indicated BEPS-IB exhibited positive antioxidant activity in scavenging superoxide radicals and reducing power. This is the first report on the structure and bioactivity of the mannoglucan from the B. exarata.
Keywords: Bullacta exarata; structure; polysaccharide; mannoglucan
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MDPI and ACS Style

Liu, D.; Liao, N.; Ye, X.; Hu, Y.; Wu, D.; Guo, X.; Zhong, J.; Wu, J.; Chen, S. Isolation and Structural Characterization of a Novel Antioxidant Mannoglucan from a Marine Bubble Snail, Bullacta exarata (Philippi). Mar. Drugs 2013, 11, 4464-4477.

AMA Style

Liu D, Liao N, Ye X, Hu Y, Wu D, Guo X, Zhong J, Wu J, Chen S. Isolation and Structural Characterization of a Novel Antioxidant Mannoglucan from a Marine Bubble Snail, Bullacta exarata (Philippi). Marine Drugs. 2013; 11(11):4464-4477.

Chicago/Turabian Style

Liu, Donghong; Liao, Ningbo; Ye, Xingqian; Hu, Yaqin; Wu, Dan; Guo, Xin; Zhong, Jianjun; Wu, Jianyong; Chen, Shiguo. 2013. "Isolation and Structural Characterization of a Novel Antioxidant Mannoglucan from a Marine Bubble Snail, Bullacta exarata (Philippi)." Mar. Drugs 11, no. 11: 4464-4477.

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