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Mar. Drugs 2013, 11(10), 3846-3860; doi:10.3390/md11103846
Article

Functional Metabolomics Uncovers Metabolic Alterations Associated to Severe Oxidative Stress in MCF7 Breast Cancer Cells Exposed to Ascididemin

1,2
Received: 18 July 2013; in revised form: 26 August 2013 / Accepted: 29 August 2013 / Published: 11 October 2013
(This article belongs to the Special Issue Marine Compounds and Inflammation)
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Abstract: Marine natural products are a source of promising agents for cancer treatment. However, there is a need to improve the evaluation of their mechanism of action in tumors. Metabolomics of the response to anti-tumor agents is a tool to reveal candidate biomarkers and metabolic targets. We used two-dimensional high-resolution magic angle spinning proton-NMR spectroscopy-based metabolomics to investigate the response of MCF7 breast cancer cells to ascididemin, a marine alkaloid and lead molecule for anti-cancer treatment. Ascididemin induced severe oxidative stress and apoptosis within 48 h of exposure. Thirty-three metabolites were quantified. Metabolic response involved downregulation of glycolysis and the tricarboxylic acid cycle, and phospholipid metabolism alterations. Candidate metabolic biomarkers of the response of breast cancer cells to ascididemin were proposed including citrate, gluconate, polyunsaturated fatty acids, glycerophospho-choline and -ethanolamine. In addition, candidate metabolic targets were identified. Overall, the response to Asc could be related to severe oxidative stress and anti-inflammatory effects.
Keywords: ascididemin; breast adenocarcinoma cells; metabolomics; biomarkers; metabolic pathway discovery; oxidative stress; inflammation ascididemin; breast adenocarcinoma cells; metabolomics; biomarkers; metabolic pathway discovery; oxidative stress; inflammation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Morvan, D. Functional Metabolomics Uncovers Metabolic Alterations Associated to Severe Oxidative Stress in MCF7 Breast Cancer Cells Exposed to Ascididemin. Mar. Drugs 2013, 11, 3846-3860.

AMA Style

Morvan D. Functional Metabolomics Uncovers Metabolic Alterations Associated to Severe Oxidative Stress in MCF7 Breast Cancer Cells Exposed to Ascididemin. Marine Drugs. 2013; 11(10):3846-3860.

Chicago/Turabian Style

Morvan, Daniel. 2013. "Functional Metabolomics Uncovers Metabolic Alterations Associated to Severe Oxidative Stress in MCF7 Breast Cancer Cells Exposed to Ascididemin." Mar. Drugs 11, no. 10: 3846-3860.


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