Next Article in Journal
Chlorella 11-Peptide Inhibits the Production of Macrophage-Induced Adhesion Molecules and Reduces Endothelin-1 Expression and Endothelial Permeability
Next Article in Special Issue
Anti-Inflammatory Activities of Natural Products Isolated from Soft Corals of Taiwan between 2008 and 2012
Previous Article in Journal
Evaluation of Passive Samplers as a Monitoring Tool for Early Warning of Dinophysis Toxins in Shellfish
Previous Article in Special Issue
Anti-Inflammatory Components of the Starfish Astropecten polyacanthus
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2013, 11(10), 3846-3860; doi:10.3390/md11103846

Functional Metabolomics Uncovers Metabolic Alterations Associated to Severe Oxidative Stress in MCF7 Breast Cancer Cells Exposed to Ascididemin

1
Faculty of Medicine, University of Auvergne-UDA, 28 Place Henri Dunant, BP 38, Clermont-Ferrand F-63001, France
2
Comprehensive Cancer Centre Jean Perrin, 58 rue Montalembert, Clermont-Ferrand F-63011, France 
Received: 18 July 2013 / Revised: 26 August 2013 / Accepted: 29 August 2013 / Published: 11 October 2013
(This article belongs to the Special Issue Marine Compounds and Inflammation)
View Full-Text   |   Download PDF [1112 KB, uploaded 24 February 2015]   |  

Abstract

Marine natural products are a source of promising agents for cancer treatment. However, there is a need to improve the evaluation of their mechanism of action in tumors. Metabolomics of the response to anti-tumor agents is a tool to reveal candidate biomarkers and metabolic targets. We used two-dimensional high-resolution magic angle spinning proton-NMR spectroscopy-based metabolomics to investigate the response of MCF7 breast cancer cells to ascididemin, a marine alkaloid and lead molecule for anti-cancer treatment. Ascididemin induced severe oxidative stress and apoptosis within 48 h of exposure. Thirty-three metabolites were quantified. Metabolic response involved downregulation of glycolysis and the tricarboxylic acid cycle, and phospholipid metabolism alterations. Candidate metabolic biomarkers of the response of breast cancer cells to ascididemin were proposed including citrate, gluconate, polyunsaturated fatty acids, glycerophospho-choline and -ethanolamine. In addition, candidate metabolic targets were identified. Overall, the response to Asc could be related to severe oxidative stress and anti-inflammatory effects. View Full-Text
Keywords: ascididemin; breast adenocarcinoma cells; metabolomics; biomarkers; metabolic pathway discovery; oxidative stress; inflammation ascididemin; breast adenocarcinoma cells; metabolomics; biomarkers; metabolic pathway discovery; oxidative stress; inflammation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Morvan, D. Functional Metabolomics Uncovers Metabolic Alterations Associated to Severe Oxidative Stress in MCF7 Breast Cancer Cells Exposed to Ascididemin. Mar. Drugs 2013, 11, 3846-3860.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top