Mar. Drugs 2012, 10(12), 2826-2845; doi:10.3390/md10122826
Article

An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes

Mei Jing Piao 1 email, Yu Jae Hyun 1 email, Suk Ju Cho 1 email, Hee Kyoung Kang 1 email, Eun Sook Yoo 1 email, Young Sang Koh 1 email, Nam Ho Lee 2 email, Mi Hee Ko 3 email and Jin Won Hyun 1,* email
1 School of Medicine, Jeju National University, Jeju 690-756, Korea 2 Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju 690-756, Korea 3 Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 699-943, Korea
* Author to whom correspondence should be addressed.
Received: 29 October 2012; in revised form: 30 November 2012 / Accepted: 5 December 2012 / Published: 14 December 2012
(This article belongs to the Special Issue Marine Algae)
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Abstract: The present study investigated the photoprotective properties of an ethanol extract derived from the red alga Bonnemaisonia hamifera against ultraviolet B (UVB)-induced cell damage in human HaCaT keratinocytes. The Bonnemaisonia hamifera ethanol extract (BHE) scavenged the superoxide anion generated by the xanthine/xanthine oxidase system and the hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2), both of which were detected by using electron spin resonance spectrometry. In addition, BHE exhibited scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) that were induced by either hydrogen peroxide or UVB radiation. BHE reduced UVB-induced apoptosis, as shown by decreased apoptotic body formation and DNA fragmentation. BHE also attenuated DNA damage and the elevated levels of 8-isoprostane and protein carbonyls resulting from UVB-mediated oxidative stress. Furthermore, BHE absorbed electromagnetic radiation in the UVB range (280–320 nm). These results suggest that BHE protects human HaCaT keratinocytes against UVB-induced oxidative damage by scavenging ROS and absorbing UVB photons, thereby reducing injury to cellular components.
Keywords: Bonnemaisonia hamifera; human keratinocytes; photoprotection; reactive oxygen species; ultraviolet B

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MDPI and ACS Style

Piao, M.J.; Hyun, Y.J.; Cho, S.J.; Kang, H.K.; Yoo, E.S.; Koh, Y.S.; Lee, N.H.; Ko, M.H.; Hyun, J.W. An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes. Mar. Drugs 2012, 10, 2826-2845.

AMA Style

Piao MJ, Hyun YJ, Cho SJ, Kang HK, Yoo ES, Koh YS, Lee NH, Ko MH, Hyun JW. An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes. Marine Drugs. 2012; 10(12):2826-2845.

Chicago/Turabian Style

Piao, Mei J.; Hyun, Yu J.; Cho, Suk J.; Kang, Hee K.; Yoo, Eun S.; Koh, Young S.; Lee, Nam H.; Ko, Mi H.; Hyun, Jin W. 2012. "An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes." Mar. Drugs 10, no. 12: 2826-2845.

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