Mar. Drugs 2012, 10(12), 2618-2633; doi:10.3390/md10122618
Article

A Sulfated-Polysaccharide Fraction from Seaweed Gracilaria birdiae Prevents Naproxen-Induced Gastrointestinal Damage in Rats

1email, 2email, 1email, 1email, 1email, 1email, 3email, 3email, 2email, 2email, 1email and 1,* email
Received: 26 September 2012; in revised form: 16 October 2012 / Accepted: 18 October 2012 / Published: 22 November 2012
(This article belongs to the Special Issue Marine Secondary Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Red seaweeds synthesize a great variety of sulfated galactans. Sulfated polysaccharides (PLSs) from seaweed are comprised of substances with pharmaceutical and biomedical potential. The aim of the present study was to evaluate the protective effect of the PLS fraction extracted from the seaweed Gracilaria birdiae in rats with naproxen-induced gastrointestinal damage. Male Wistar rats were pretreated with 0.5% carboxymethylcellulose (control group—vehicle) or PLS (10, 30, and 90 mg/kg, p.o.) twice daily (at 09:00 and 21:00) for 2 days. After 1 h, naproxen (80 mg/kg, p.o.) was administered. The rats were killed on day two, 4 h after naproxen treatment. The stomachs were promptly excised, opened along the greater curvature, and measured using digital calipers. Furthermore, the guts of the animals were removed, and a 5-cm portion of the small intestine (jejunum and ileum) was used for the evaluation of macroscopic scores. Samples of the stomach and the small intestine were used for histological evaluation, morphometric analysis and in assays for glutathione (GSH) levels, malonyldialdehyde (MDA) concentration, and myeloperoxidase (MPO) activity. PLS treatment reduced the macroscopic and microscopic naproxen-induced gastrointestinal damage in a dose-dependent manner. Our results suggest that the PLS fraction has a protective effect against gastrointestinal damage through mechanisms that involve the inhibition of inflammatory cell infiltration and lipid peroxidation.
Keywords: sulfated polysaccharide; gastrointestinal damage; naproxen; antioxidant activity
PDF Full-text Download PDF Full-Text [596 KB, uploaded 22 November 2012 13:25 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Silva, R.O.; Santana, A.P.M.; Carvalho, N.S.; Bezerra, T.S.; Oliveira, C.B.; Damasceno, S.R.B.; Chaves, L.S.; Freitas, A.L.P.; Soares, P.M.G.; Souza, M.H.L.P.; Barbosa, A.L.R.; Medeiros, J.-V.R. A Sulfated-Polysaccharide Fraction from Seaweed Gracilaria birdiae Prevents Naproxen-Induced Gastrointestinal Damage in Rats. Mar. Drugs 2012, 10, 2618-2633.

AMA Style

Silva RO, Santana APM, Carvalho NS, Bezerra TS, Oliveira CB, Damasceno SRB, Chaves LS, Freitas ALP, Soares PMG, Souza MHLP, Barbosa ALR, Medeiros J-VR. A Sulfated-Polysaccharide Fraction from Seaweed Gracilaria birdiae Prevents Naproxen-Induced Gastrointestinal Damage in Rats. Marine Drugs. 2012; 10(12):2618-2633.

Chicago/Turabian Style

Silva, Renan O.; Santana, Ana P.M.; Carvalho, Nathalia S.; Bezerra, Talita S.; Oliveira, Camila B.; Damasceno, Samara R.B.; Chaves, Luciano S.; Freitas, Ana L.P.; Soares, Pedro M.G.; Souza, Marcellus H.L.P.; Barbosa, André L.R.; Medeiros, Jand-Venes R. 2012. "A Sulfated-Polysaccharide Fraction from Seaweed Gracilaria birdiae Prevents Naproxen-Induced Gastrointestinal Damage in Rats." Mar. Drugs 10, no. 12: 2618-2633.


Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert