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Pharmaceuticals 2013, 6(4), 510-521; doi:10.3390/ph6040510
Review

Delivery of RNAi-Based Oligonucleotides by Electropermeabilization

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Received: 31 December 2012; in revised form: 19 March 2013 / Accepted: 27 March 2013 / Published: 10 April 2013
(This article belongs to the Special Issue RNAi-Based Therapeutics)
View Full-Text   |   Download PDF [622 KB, uploaded 10 April 2013]
Abstract: For more than a decade, understanding of RNA interference (RNAi) has been a growing field of interest. The potent gene silencing ability that small oligonucleotides have offers new perspectives for cancer therapeutics. One of the present limits is that many biological barriers exist for their efficient delivery into target cells or tissues. Electropermeabilization (EP) is one of the physical methods successfully used to transfer small oligonucleotides into cells or tissues. EP consists in the direct application of calibrated electric pulses to cells or tissues that transiently permeabilize the plasma membranes, allowing efficient in vitro and in vivo. cytoplasmic delivery of exogenous molecules. The present review reports on the type of therapeutic RNAi-based oligonucleotides that can be electrotransferred, the mechanism(s) of their electrotransfer and the technical settings for pre-clinical purposes.
Keywords: electroporation; siRNA; miRNA; LNA; siLNA electroporation; siRNA; miRNA; LNA; siLNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chabot, S.; Pelofy, S.; Teissié, J.; Golzio, M. Delivery of RNAi-Based Oligonucleotides by Electropermeabilization. Pharmaceuticals 2013, 6, 510-521.

AMA Style

Chabot S, Pelofy S, Teissié J, Golzio M. Delivery of RNAi-Based Oligonucleotides by Electropermeabilization. Pharmaceuticals. 2013; 6(4):510-521.

Chicago/Turabian Style

Chabot, Sophie; Pelofy, Sandrine; Teissié, Justin; Golzio, Muriel. 2013. "Delivery of RNAi-Based Oligonucleotides by Electropermeabilization." Pharmaceuticals 6, no. 4: 510-521.


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