Open AccessThis article is
- freely available
Design of siRNA Therapeutics from the Molecular Scale
Department of Chemical Engineering and Materials Science, Michigan State University, 428 S. Shaw Lane, Room 2527, East Lansing, MI 48824, USA
* Author to whom correspondence should be addressed.
Received: 24 January 2013; in revised form: 27 February 2013 / Accepted: 13 March 2013 / Published: 25 March 2013
Abstract: While protein-based therapeutics is well-established in the market, development of nucleic acid therapeutics has lagged. Short interfering RNAs (siRNAs) represent an exciting new direction for the pharmaceutical industry. These small, chemically synthesized RNAs can knock down the expression of target genes through the use of a native eukaryotic pathway called RNA interference (RNAi). Though siRNAs are routinely used in research studies of eukaryotic biological processes, transitioning the technology to the clinic has proven challenging. Early efforts to design an siRNA therapeutic have demonstrated the difficulties in generating a highly-active siRNA with good specificity and a delivery vehicle that can protect the siRNA as it is transported to a specific tissue. In this review article, we discuss design considerations for siRNA therapeutics, identifying criteria for choosing therapeutic targets, producing highly-active siRNA sequences, and designing an optimized delivery vehicle. Taken together, these design considerations provide logical guidelines for generating novel siRNA therapeutics.
Keywords: siRNA therapeutic; RNAi; liver cancer; siRNA design; delivery vehicle design
Citations to this Article
Cite This Article
MDPI and ACS Style
Angart, P.; Vocelle, D.; Chan, C.; Walton, S.P. Design of siRNA Therapeutics from the Molecular Scale. Pharmaceuticals 2013, 6, 440-468.
Angart P, Vocelle D, Chan C, Walton SP. Design of siRNA Therapeutics from the Molecular Scale. Pharmaceuticals. 2013; 6(4):440-468.
Angart, Phillip; Vocelle, Daniel; Chan, Christina; Walton, S. P. 2013. "Design of siRNA Therapeutics from the Molecular Scale." Pharmaceuticals 6, no. 4: 440-468.