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Sensors 2009, 9(8), 6185-6199; doi:10.3390/s90806185
Article

Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres

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Received: 10 June 2009 / Revised: 23 July 2009 / Accepted: 3 August 2009 / Published: 5 August 2009
(This article belongs to the Section Biosensors)
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Abstract

Novel magnetic Fe3O4/chitosan (CS) microspheres were prepared using magnetic Fe3O4 nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin (Hb) immobilization method, an innovative biosensor with a Fe3O4/CS-Hb-Fe3O4/CS “sandwich” configuration was constructed. This biosensor had a fast (less than 10 s) response to H2O2 and excellent linear relationships were obtained in the concentration range of 5.0 × 10-5 to 1.8 × 10-3 M and 1.8 × 10-3 to 6.8 × 10-3 M with a detection limit of 4.0 × 10-6 M (s/n = 3) under the optimum conditions. The apparent Michaelis-Menten constant Km was 0.29 mM and it showed the excellent biological activity of the fixed Hb. Moreover, the biosensor had long-time stability and good reproducibility. The method was used to determine H2O2 concentration in real samples.
Keywords: hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tan, X.-C.; Zhang, J.-L.; Tan, S.-W.; Zhao, D.-D.; Huang, Z.-W.; Mi, Y.; Huang, Z.-Y. Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres. Sensors 2009, 9, 6185-6199.

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