Next Article in Journal
Fabrication and Performance of MEMS-Based Pressure Sensor Packages Using Patterned Ultra-Thick Photoresists
Previous Article in Journal
Monitoring System for Farming Operations with Wearable Devices Utilized Sensor Networks
Article Menu

Export Article

Open AccessArticle
Sensors 2009, 9(8), 6185-6199; doi:10.3390/s90806185

Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres

College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China
*
Author to whom correspondence should be addressed.
Received: 10 June 2009 / Revised: 23 July 2009 / Accepted: 3 August 2009 / Published: 5 August 2009
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [435 KB, uploaded 21 June 2014]   |  

Abstract

Novel magnetic Fe3O4/chitosan (CS) microspheres were prepared using magnetic Fe3O4 nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin (Hb) immobilization method, an innovative biosensor with a Fe3O4/CS-Hb-Fe3O4/CS “sandwich” configuration was constructed. This biosensor had a fast (less than 10 s) response to H2O2 and excellent linear relationships were obtained in the concentration range of 5.0 × 10-5 to 1.8 × 10-3 M and 1.8 × 10-3 to 6.8 × 10-3 M with a detection limit of 4.0 × 10-6 M (s/n = 3) under the optimum conditions. The apparent Michaelis-Menten constant Km was 0.29 mM and it showed the excellent biological activity of the fixed Hb. Moreover, the biosensor had long-time stability and good reproducibility. The method was used to determine H2O2 concentration in real samples. View Full-Text
Keywords: hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tan, X.-C.; Zhang, J.-L.; Tan, S.-W.; Zhao, D.-D.; Huang, Z.-W.; Mi, Y.; Huang, Z.-Y. Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres. Sensors 2009, 9, 6185-6199.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top