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Sensors 2009, 9(8), 6185-6199; doi:10.3390/s90806185
Article

Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres

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College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China
* Author to whom correspondence should be addressed.
Received: 10 June 2009 / Revised: 23 July 2009 / Accepted: 3 August 2009 / Published: 5 August 2009
(This article belongs to the Section Biosensors)
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Abstract

Novel magnetic Fe3O4/chitosan (CS) microspheres were prepared using magnetic Fe3O4 nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin (Hb) immobilization method, an innovative biosensor with a Fe3O4/CS-Hb-Fe3O4/CS “sandwich” configuration was constructed. This biosensor had a fast (less than 10 s) response to H2O2 and excellent linear relationships were obtained in the concentration range of 5.0 × 10-5 to 1.8 × 10-3 M and 1.8 × 10-3 to 6.8 × 10-3 M with a detection limit of 4.0 × 10-6 M (s/n = 3) under the optimum conditions. The apparent Michaelis-Menten constant Km was 0.29 mM and it showed the excellent biological activity of the fixed Hb. Moreover, the biosensor had long-time stability and good reproducibility. The method was used to determine H2O2 concentration in real samples.
Keywords: hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Tan, X.-C.; Zhang, J.-L.; Tan, S.-W.; Zhao, D.-D.; Huang, Z.-W.; Mi, Y.; Huang, Z.-Y. Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4/Chitosan Core-Shell Microspheres. Sensors 2009, 9, 6185-6199.

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